Zero Hunger (SDG 2), Good Health & Well-being (SDG 3)
Induced production of putative silenced secondary metabolite compounds using chemical approaches
Winnifred Duk*, Matthew Landu Papua New Guinea
Mushrooms secrete secondary metabolites allowing them to disseminate and survive adverse environmental conditions. It is hypothesized that mycelia of Corpinus lagopus treated with small molecule (chemical elicitors) will have enhanced production of secondary metabolites. Coprinus lagopus (C.lagopus) mycelia were cultured on solid Potato Dextrose Agar for all the treatments. In experimental subcultures samples were treated with either Trichostatin A , 5-azacytidine, or left untreated. Compound extraction was done with either ethyl acetate or methanol. Each sample was spotted using capillary tubes on Thin Layer Chromatographic (TLC) plates and tested using both reverse-phase and normal- phase TLC. Isolation of putative secondary metabolite compound was done using reverse-phase High Performance Liquid Chromatography (HPLC). General observation of phenotypic characteristics of daily mycelia colony growth showed white, cottony texture. Additional putative compounds were isolated by HPLC and qualitatively displayed on a chromatogram. It is probable that these putative compounds were produced as a result of the activation of the silent secondary metabolite gene clusters through remodeling of the chromatin. Key Words: Fungi, secondary metabolites, silent gene clusters, High Performance Liquid Chromatography.
P14
© The Author(s), 2023
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