Chelex-based PCR sex identification of Lynx rufus Muscle Tissues
Deagan Wiebel (Not Pictured)
Ecosystem Restoration and Management
DNA extraction is a useful method in identifying the sex of animals using muscle tissues, hair samples, and even scat. The issue is that if not bought in bulk, singular extension kits can charge a large sum to run a small amount of samples. These kits often also take upwards of three days to clean the DNA. Pilgrim et al. 2004, utilizes QIAamp DNA stool mini kits and primers targeting the amelogenin region of non-somatic chromosomes to differentiate male and female tissues of Lynx rufus via PCR. These results can be replicated using DNeasy extraction kits on Lynx rufus muscle tissues. DNeasy DNA extraction, although producing high quality extractions, is a rather costly and labor-intensive process. Chelex extraction is another method of obtaining DNA that, although producing less clean DNA, costs less than DNeasy kits, requires less labor from the technician, and can produce extractions at a larger, more scalable size. In this study we used primers from Pilgrim et. al. 2004 to test the effectiveness of chelex-based DNA extractions for sex identification of Lynx rufus muscle tissues. This study aims to find if chelex DNA extraction can be applied to the methods from Pilgrim et al. 2004 for similar results in the sex identification of Lynx rufus . This information can be applied to help cut costs and extraction time lengths in resulting Lynx spp. population studies.
Advisors: Amy Springer, Ph.D.
Subject Area: Wildlife Genetics
Type: Poster
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UW-Stevens Point College of Natural Resources
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