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Table of Contents HPLC COLUMNS
METAL FREE COLUMNS Scepter Series............................................................................................A-86 G Series......................................................................................................A-87 Mastro™ Series.........................................................................................A-88
Column Selection Guide.............................................................................A-2 Selection by USP Code................................................................................A-7
CORESHELL COLUMN VELOX SERIES Velox C18...................................................................................................A-10 Velox SP-C18..............................................................................................A-10 Velox Biphenyl..........................................................................................A-12 Velox PFPP.................................................................................................A-12 Velox HILIC................................................................................................A-13 ORGANIC SILICA HYBRID SCEPTER SERIES Scepter C18...............................................................................................A-16 Scepter HD-C18.........................................................................................A-17 Scepter C8.................................................................................................A-18 Scepter PFPP..............................................................................................A-19 Scepter Pheny I.........................................................................................A-20 Scepter Diol-HILIC.....................................................................................A-21 Scepter C4.................................................................................................A-22 ARATA SERIES Arata C18..................................................................................................A-25 Arata Peptide C18.....................................................................................A-26
SFC COLUMNS UC Series....................................................................................................A-90
DEDICATED COLUMNS Cannabis Analysis.....................................................................................A-94 Analysis of Amino Acids...........................................................................A-95 Analysis of Sugar and Organic Acid........................................................A-96 Analysis of Organic Acids.........................................................................A-98 Analysis of Nucleotides, Oligonucleotides and Proteins........................A-99 Intact Amino Acid Analysis....................................................................A-100 ION CHROMATOGRAPHY COLUMNS IC Series...................................................................................................A-101 Ultron PS-80H..........................................................................................A-105 PRETREATMENT COLUMNS MAYI Series.............................................................................................A-106 SPC Series................................................................................................A-108 CHIRAL COLUMNS ULTRON SERIES Ultron ES-OVM........................................................................................A-110 Ultron ES-OVM-C....................................................................................A-110 Ultron ES-OVM-3....................................................................................A-110 Ultron ES-PEPSIN.....................................................................................A-111 Ultron ES-BSA..........................................................................................A-111 Ultron ES-CD...........................................................................................A-112 Ultron ES-PhCD.......................................................................................A-112 LIGAND EXCHANGE COLUMNS ULTRON SERIES Ultron PS-80N..........................................................................................A-113 Ultron PS-80C..........................................................................................A-113 Ultron PS-80P..........................................................................................A-113 Ultron CI..................................................................................................A-114 Ultron CL.................................................................................................A-114 ULTRON SERIES Ultron VX-SIL...........................................................................................A-115 Ultron VX-ODS........................................................................................A-116 Ultron VX-Octyl.......................................................................................A-117 Ultron AF-HILIC-DA.................................................................................A-118 STR-ODS SERIES STR-ODS-II. ..............................................................................................A-119 STR ODS-M..............................................................................................A-120 MOBILE PHASE CLEANER FOR UHPLC/HPLC Ghost Trap DS/DS-HP..............................................................................A-121 GLC Suction Filter 2................................................................................A-122
GENERAL PURPOSE COLUMNS XR-ODS SERIES
XR-ODS......................................................................................................A-28 XR-C8.........................................................................................................A-28 XR-Phenyl..................................................................................................A-28 XR SIL........................................................................................................ A-28 XR-ODS II...................................................................................................A-30 XR-ODS III..................................................................................................A-30 VP SERIES VP-ODS..................................................................................................... A-32 VP-C8........................................................................................................ A-32 VP-Phenyl................................................................................................. A-32
MAqC-ODS ................................................................................................A-36
G SERIES GIST C18................................................................................................... A-38 GIST C18-AQ............................................................................................. A-40 GISS C18.................................................................................................... A-42 GIS C18..................................................................................................... A-44 GIS C18-P.................................................................................................. A-46 GIS RP-Shield............................................................................................ A-48 GWS C18................................................................................................... A-49 GIST C8..................................................................................................... A-50 GIST Phenyl.............................................................................................. A-52 GIST Phenyl-Hexyl.................................................................................... A-54 GIST PFPP...................................................................................................A-56 GIS HILIC....................................................................................................A-58 GIST NH2...................................................................................................A-60 GIST Amide................................................................................................A-62 GIS CN........................................................................................................A-64 Solar C18...................................................................................................A-66 Solar C8.................................................................................................... A-66 FC-ODS.......................................................................................................A-67 PREPARATIVE COLUMNS PREP Series............................................................................................... A-69 G Series..................................................................................................... A-69 UC Series....................................................................................................A-71 Ultron VX Series........................................................................................A-72 STR-ODS Series..........................................................................................A-72
FITTINGS ..................................................................................................A-123
VIALS AND ACCESSORIES VIALS ........................................................................................................... B-2 SOLVENT KITS . .......................................................................................... B-19 SAMPLE PREPARATION ............................................................................. B-21 GC COLUMNS AND ACCESSORIES GC COLUMN CROSS-REFERENCE . .............................................................. C-4 GC CAPILLARY COLUMNS . ......................................................................... C-6 GC PACKED COLUMNS . ............................................................................ C-51 SYRINGES .................................................................................................. C-57 GLASS INSERT . .......................................................................................... C-67 FERRULES . ................................................................................................. C-70 SEPTUMS ................................................................................................... C-72 SPME ARROW ........................................................................................... C-73 SPME FIBER . .............................................................................................. C-74
MIXED-MODE COLUMNS Shimadzu Redual™ Columns...................................................................A-73
BIO LC COLUMNS Scepter C4.................................................................................................A-74 Bio Series...................................................................................................A-76 Arata Peptide C18.....................................................................................A-77 Bio HIC--Butyl............................................................................................A-78 MICROSCALE COLUMNS MC C18......................................................................................................A-79 MC PLONAS Series....................................................................................A-80 Trap Columns............................................................................................A-82 SIZE EXCLUSION CHROMATOGRAPHY COLUMNS GPC Series..................................................................................................A-83 Diol Series..................................................................................................A-85
GAS FLTRATION . ..............................................................................D-1
SPECTRUM CONSUMABLES .................................................... E-1
APPENDIX I..................................................................................................F-1
UHPLC/HPLC Columns and LC Accessories
Column Selection Guide
Selection by Support Materials
Shim-pack XR, VP, MAqC-ODS, G Series, FC-ODS, Velox Series, Arata C18 Shim-pack UC Series Shim-pack Diol Series Shim-pack WAX/WCX Series
Silica Gel Type
Packing Materials
Organic Silica Hybrid
Shim-pack Scepter Series
Shim-pack GPC Series Shim-pack IC Series Shim-pack Amino, SCR, ISA/ISC Series
Polymer Type
Selection by Separation Modes
ODS/C18 (Shim-pack XR, VP, MAqC, G, FC Series, Velox, Scepter) Shim-pack GIST C18-AQ Shim-pack GIS C18-P Shim-pack GIS RP-Shield C8 (Shim-pack XR, VP, G Series) Phenyl (Shim-pack XR, VP, G Series) Shim-pack GIST Phenyl-Hexyl Shim-pack Velox Biphenyl Shim-pack Velox PFPP, Shim-pack Scepter PFPP
Low molecular- weight compounds
Reversed Phase
Shim-pack GIS CN.........................................................................Steroid hormones Shim-pack XR-SIL................................................................................Phospholipids Shim-pack GIST NH2.........................................................Saccharides, tocopherols
Normal Phase
Silica Gel Type
HILIC Mode
Shim-pack GIS HILIC, Velox HILIC, Scepter HILIC.........................Polar compounds
Shim-pack WAX (weakly basic anion exchange)..................Nucleotides, proteins Shim-pack WCX (weakly acidic cation exchange).......................................Proteins
Ion Exchange
Shim-pack Diol Series...................................................................................Proteins Shim-pack Bio Diol Series...........MAb, oligonuclestides, carbonydrates, proteins
Gel Filtration
Shim-pack IC Series.............................................................................Inorganic ions Shim-pack Amino, ISC Series............................................Amino acids, guanidines Shim-pack ISA Series...............................................................................Saccharides Shim-pack Bio IEX..........................Peptides, oligonuclestides, biopharmaceutical, proteins, nucleic acids
Ion Exchange
Polymer Type
Shim-pack SCR-101H, SCR-102H......................................................... Organic acids Shim-pack Fast-OA...............................................................................Organic acids
Ion Exclusion
GPC
Shim-pack GPC Series....................................................... Synthetic high polymers
Pg A-2
Selection by Column Particle Sizes
1.6 µm, 2.2 µm
Shim-pack XR-ODS III, Arata C18
High-Speed Analysis
1.8 µm, 2.7 µm
Shim-pack Velox Series
1.9 µm, 2 µm, 2.2 µm, 3 µm
Analytical Columns
Shim-pack G, XR Series, Scepter Series
Conventional Analysis
Shim-pack VP, MAqC-ODS, G Series, FC-ODS, Arata C18, Velox Series, Scepter Series
≥ 3 µm
HPLC Columns
5 µm, 10 µm
Shim-pack G Series, Scepter Series
Preparative Columns
15 µm
Shim-pack PREP Series
Selection of ODS columns
Shim-pack XR-ODS (Pressure tolerance: 35MPa)..................A-28 Shim-pack XR-ODS II (Pressure tolerance: 60MPa)...............A-30 Shim-pack XR-ODS III (Pressure tolerance: 100MPa)............A-30
With Certificate of Compliance for Validation
Shim-pack Scepter C18 / HD-C18 (pH range: 1 - 12).............A-16 Shim-pack GIST/GIST-HP C18 (pH range: 1 - 10)....................A-38
Wider pH Range
Rapid Separation and Wider pH Range
High-Speed Analysis
Shim-pack GISS/GISS-HP C18 (pH range: 1 - 10)....................A-42
Shim-pack Scepter C18 / HD-C18............................................A-16 Shim-pack GIS/GIS-HP C18......................................................A-44
Stronger Retentivity
Strong Retentivity of Highly Polar Compounds
Shim-pack GIST/GIST-HP C18-AQ............................................A-38
With Certificate of Compliance for Validation
Shim-pack VP-ODS..................................................................A-32
Shim-pack Scepter C18 / HD-C18 (pH range: 1 - 12).............A-16 Shim-pack GIST C18 (pH range: 1 - 10)..................................A-38
Wider pH Range
Shim-pack GIS C18..................................................................A-44 Shim-pack FC-ODS (pH range: 1.5 - 9)...................................A-67 Shim-pack Scepter HD C18.....................................................A-17
Stronger Retentivity
Conventional Analysis
Higher Steric Selectivity
Shim-pack GIS C18-P...............................................................A-46
Different Elution Pattern
Shim-pack GIS RP-Shield.........................................................A-48
Higher Cost-Performance
Shim-pack GWS C18................................................................A-49
Strong Retentivity of Basic Compounds
Shim-pack MAqC-ODS............................................................A-36
Pg A-3
Column Selection Guide
Column Selection by Target Compounds
Anion Exchange
High Polar Acid Compounds
Ion Exclusion
High Polar Neutral Compounds
Insoluble in Ethanol
Normal Phase
High Polar Basic Compounds
Cation Exchange
Soluble in Water
Mid-Polar Neutral Compounds
Soluble in Ethanol
Insoluble in Acid Water
Low Polar Acid Salts
Low Polar Basic Salts
Insoluble in Basic Water
Target Compounds
Reversed Phase
Low Polar Basic Compounds
Soluble in Acid Water
Low Polar Acid Compounds
Soluble in Basic Water
Insoluble in Water
Mid-Polar Neutral Compounds
Soluble in Ethanol
Normal Phase
Insoluble in Ethanol
Polymer Compounds
Size Exclusion
Column Selection by Target Compounds
Saturation Aliphatic Carboxylic Acids
Shim-pack Velox C18.......A-10 Shim-pack Scepter C18....A-16 Shim-pack GIST C18.........A.38
Reversed Phase
Non-saturation Aliphatic Carboxylic Acids
Aliphatic Carboxylic Acids
Shim-pack SCR-101H........A-96 Shim-pack SCR-102H........A-96
Ion Exclusion
Hydroxy and Keto Carboxylic Acids
Carboxylic Acids
Shim-pack IC-A1.............A-104 Shim-pack WAX-1............A-99
Anion Exchange
Shim-pack Velox C18.......A-10 Shim-pack Scepter C18....A-16 Shim-pack GIST C18.........A-38
Aromatic Carboxylic Acids
Reversed Phase
Pg A-4
Column Selection by Target Compounds
Shim-pack Scepter Dio-HILIC.................................A-21
HILIC Mode
Free Amino Acids
Shim-pack AMINO-LI.............................................A-95 (Post-column derivation fluorescing with OPA)
Cation Exchange
Shim-pack AMINO-NA...........................................A-95 (Post-column derivation fluorescing with OPA)
Cation Exchange
Amino Acids
Shim-pack Velox C18.............................................A-10 Shim-pack Scepter C18..........................................A-16 Shim-pack GIST C18...............................................A-38
Reversed Phase (Pre-column derivatization)
Protein-hydrolyzed Amino Acids
Shim-pack Velox C18.............................................A-10 Shim-pack Scepter C18..........................................A-16 Shim-pack GIST C18...............................................A-38
Reversed Phase + Ion Pair
Shim-pack Velox C18.............................................A-10 Shim-pack Scepter C18..........................................A-16 Shim-pack Arata Peptide C18...............................A-27 Shim-pack GIST C18...............................................A-38 Shim-pack GISS C18...............................................A-42
Peptides
Reversed Phase
Peptides Proteins
Shim-pack Scepter C4............................................A-22
Reversed Phase
Shim-pack Diol Series............................................A-85 Shim-pack Bio Diol.................................................A-77
Gel Filtration
Shim-pack Bio IEX SP/SP-NP...................................A-77 Shim-pack WCX-1..................................................A-99
Cation Exchange
Proteins
Shim-pack Bio IEX Q/Q-NP.....................................A-77 Shim-pack WAX-2..................................................A-99
Anion Exchange
Hydrophobic Interaction
Shim-pack Bio HIC................................................A-133
Shim-pack GIST NH2..............................................A-60
HILIC Mode
Monosaccharides
Gel Filtration + Ligand Exchange
Shim-pack SCR-101N/C/P........................................A-96
Mono- to tri-saccharides
Shim-pack ISA-07...................................................A-97
Anion Exchange
Sugars
Oligosaccharides
Shim-pack GIST NH2..............................................A-60
HILIC Mode
Shim-pack Velox C18.............................................A-10 Shim-pack Scepter C18..........................................A-16 Shim-pack GIST C18...............................................A-38
Derivatized Sugars
Reversed Phase
Pg A-5
Column Selection Guide
Column Selection by Target Compounds
Shim-pack Velox C18/SP-C18......A-10 Shim-pack Scepter......................A-16 Shim-pack GIST C18....................A-38
Mixture of Water-soluble / Fat-soluble vitamins
Reversed Phase
Vitamin C
Shim-pack GIST NH2...................A-60
HILIC Mode
Water-soluble Vitamins
Shim-pack Scepter C18...............A-16 Shim-pack MAqC-ODS................A-36
Reversed Phase
Vitamins
Others
HILIC Mode
Shim-pack Scepter Dio-HILIC......A-21
Shim-pack Velox..........................A-10 Shim-pack Scepter C18...............A-16 Shim-pack GIST C18....................A-38
Vitamin A, K
Reversed Phase
Fat-soluble Vitamins
Shim-pack GIST NH2...................A-60
Vitamin D, E
Normal Phase
Fat-soluble Vitamins
Water-soluble Vitamins
UV (325 nm) RF (Ex. 340 nm, Em. 460 nm)
UV (270 nm) RF (Post-column derivatization) UV (270 nm) RF (Post-column derivatization)
Vitamin A (retinol)
Vitamin B1 (thiamin)
Vitamin D
UV (265 nm)
Vitamin B2 (riboflavin)
UV (295 nm) RF (Ex. 295 nm, Em. 325 nm) UV (250 nm) RF (Post-column derivatization)
Vitamin E (tocopherol)
Vitamin B3 (niacin)
UV (260 nm) UV (205 nm) UV (290 nm) UV (280 nm) UV (210 nm) RI UV (245 nm) UV (210 nm) UV (280 nm) UV (265 nm)
Vitamin B5 (pantothenic acid)
Vitamin K
Vitamin B6 (pyridoxine)
Vitamin B12 (cyanocobalamine)
Vitamin BT (carnitine)
Vitamin C (ascorbic acid)
Vitamin H (biotin)
Vitamin M (folic acid) Vitamin P (hesperidin)
* The above wavelength values are approximate values. It is possible to be influenced by the state of mobile phase.
Shim-pack Bio IEX SP-NP/SP........A-77 Shim-pack WCX-1.......................A-99
Cation Exchange
Shim-pack Velox C18/SP-C18......A-10 Shim-pack Scepter C18/HD-C18..A-16 Shim-pack GIST C18....................A-38 Shim-pack Velox HILIC................A-13 Shim-pack Scepter Diol HILIC.....A-21 Shim-pack GIS HILIC....................A-58
Reversed Phase
Nucleic Acid Bases and Nucleosides
HILIC Mode
Shim-pack Bio IEX Q/Q-NP..........A-77 Shim-pack WAX-1.......................A-99
Anion Exchange
Nucleic Acids
Nucleotides
Shim-pack Velox..........................A-10 Shim-pack Scepter C18...............A-16 Shim-pack GIST C18....................A-38
Reversed Phase
Gel Filtration
Shim-pack Bio Diol......................A-77 Shim-pack Diol Series.................A-85
DNA and RNA
Shim-pack Bio IEX Q/Q-NP..........A-77 Shim-pack WAX-2.......................A-99
Anion Exchange
Pg A-6
Selection by USP
USP Code
Shim-pack HPLC Columns
Packing
Page
A-10 A-11 A-16 A-17 A-25 A-28 A-30 A-30 A-32 A-38 A-40 A-42 A-44 A-46 A-48 A-49 A-66 A-67 A-90 A-88
Shim-pack Velox C18 Shim-pack Velox SP-C18 Shim-pack Scepter C18 Shim-pack Scepter HD-C18 Shim-pack Arata C18 Shim-pack XR-ODS Shim-pack XR-ODS II Shim-pack XR-ODS III Shim-pack VP-ODS Shim-pack GIST C18 Shim-pack GIST C18-AQ
L1 Octadecyl silane chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.
Shim-pack GISS C18 Shim-pack GIS C18
Shim-pack GIS C18-P Shim-pack GIS RP-Shield Shim-pack GWS C18 Shim-pack Solar C18
Shim-pack FC-ODS Shim-pack UC GIS II Mastro2™ C18 ULTRON VX-ODS
A-116 A-119 A-120
STR ODS-II STR ODS-M
A-13 A-28 A-90 A-91
Shim-pack Velox HILIC
Shim-pack XR-SIL Shim-pack UC Sil Shim-pack UC Sil II
L3
Porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.
A-115
ULTRON VX-SIL
A-18 A-28 A-33 A-50 A-66
Shim-pack Scepter C8 Shim-pack XR-C8 Shim-pack VP-C8 Shim-pack GIST C8 Shim-pack Solar C8 ULTRON VX-Octyl Shim-pack GIST NH2 Shim-pack UC NH2 Shim-pack GIS CN Shim-pack UC CN
L7 Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.
A-117
A-60 A-90 A-64 A-90 A-12 A-20 A-28 A-32 A-52 A-54 A-90 A-96
L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod.
L10 Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.
Shim-pack Velox Biphenyl Shim-pack Scepter Phenyl Shim-pack XR-Phenyl Shim-pack VP-Phenyl Shim-pack GIST Phenyl Shim-pack GIST Phenyl-Hexyl
L11 Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.
Shim-pack UC Phenyl Shim-pack SCR-101H
L17 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 µm in diameter. L19 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 5 - 15 µm in diameter.
A-105
ULTRON PS-80H
A-96
Shim-pack SCR-101C
A-113
ULTRON PS-80C
Shim-pack Scepter Diol-HILIC A-21 Shim-pack GIS HILIC A-58 Shim-pack UC Diol A-90 Shim-pack UC Diol II A-91 Shim-pack Diol-150 A-85 Shim-pack Diol-300 A-85 Shim-pack Bio Diol A-77
L20 Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.
A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 µm in diameter.
A-83
L21
Shim-pack GPC Series
A-101
Shim-pack IC-C1
A-95 A-95 A-97
Shim-pack AMINO-LI Shim-pack AMINO-NA
L22 A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5 - 15 µm in diameter.
Shim-pack ISC Shim-pack IC-A1
L23 An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quartenary ammonium groups, 7 - 12 µm in size.
A-104
Butyl silane chemically bonded to totally porous silica particles, 1.5 to 10 μm in diameter.
A-22
L26
Shim-pack Scepter C4
L33 Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability. L34 Strong cation exchange resin consisting of sulfonated crosslinked styrene-divinylbenzene copolymer in the lead form, 9μm in diameter.
A-77
Shim-pack Bio Diol
A-113
ULTRON PS-80P
A-12 A-19 A-56 A-88
Shim-pack Velox PFPP Shim-pack Scepter PFPP Shim-pack GIST PFPP Mastro2™ PFP ULTRON ES-CD ULTRON ES-PhCD ULTRON ES-OVM Shim-pack SCR-101N
L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 μm in diameter.
A-112 A-112 A-110
Beta cyclodextrin bonded to porous silica particles, 5 to 10 μm in diameter.
L45
L57 A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 μm in diameter, with a pore size of 120 Å.
A-96
L58 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 µm diameter.
A-113
ULTRON PS-80N
A-85 A-85 A-77
Shim-pack Diol-150 Shim-pack Diol-300 Shim-pack Bio Diol
L59 Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is spherical 1.5 - 10 µm, silica or hybrid packing with a hydrophilic coating. L68 Spherical, porous silica, 10 μm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped. L101 Cholesteryl groups bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 μm in diameter, or a monolithic rod.
A-62
Shim-pack GIST Amide
A-91 A-91
Shim-pack UC-Choles Shim-pack UC-Triazole
Triazole groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter.
L104
Pg A-7
Core Shell Columns
Maximize LC Separation Performance with Core Shell Technology
Designed to maximize performance of LC systems, Shimadzu’s Shim-pack Velox columns with core shell technology enable you to achieve increased separations and faster analysis times on any LC platform. Whether developing a high efficiency LC separation method, transferring an existing method for increased throughput while maintaining resolution, or are trying to improve the resolution of a complex separation, Shim-pack Velox columns will satisfy your needs. Column ruggedness is critical to any LC analysis and Shim-pack Velox core-shell columns deliver excellent column lifetime for even the most challenging sample matrices.
Shim-pack Velox column will deliver Increased resolution with maximum efficiency g improving separation and detection Faster separation without sacrificing performance g maximizing laboratory productivity and reducing cost of analysis Increased sample throughput g reducing overall analysis time Superior ruggedness g reducing cost of analysis Excellent reproducibility g maintaining analysis and data integrity Column Selection Guide for Different LC Platforms Column particle sizes and column volumes affect chromatography results significantly if the column configuration does not match the LC system. As column particle size is reduced, or the column volume (ID and/or the length of the columns) decreases, the necessity for a lower dispersion system is increased. Choosing the optimal column configuration for your LC system allows you to achieve improved chromatography. The following table summarizes the starting recommendations of column configuration for each LC system.
Prominence
Prominence-i plus
Nexera XR
Nexera-i plus
Nexera X2
LC System
HPLC
UHPLC-like
UHPLC
Particle size Column I.D.
2.7 µm & 5 µm
2.7 µm
1.8 µm & 2.7 µm
4.6 mm (3.0 mm)
3.0 mm (2.1 mm)
2.1 mm 150 mm
Column Length
100-250 mm
50-100 mm
Pg A-8
Column Chemistries Combining highly efficient core shell particle technology with a wide range of surface chemistries provides you with the best opportunity for optimal resolution. With different chemistry characteristics, Shim-pack Velox columns are suitable for use in a wide variety of applications and challenging separations.
SP-C18
C18
Biphenyl
PFPP
HILIC
USP Classification
L1
L1
L11
L43
L3
Sterically protected C18
Pentafluorophenyl propyl
Ligand type
C18
Biphenyl
None
Particle Size (µm)
1.8, 2.7, 5
1.8, 2.7, 5
1.8, 2.7, 5
1.8, 2.7, 5
2.7
Pore size (Å)
90
90
90
90
90
125 m 2 /g 130 m 2 /g 100 m 2 /g
125 m 2 /g 130 m 2 /g 100 m 2 /g
125 m 2 /g 130 m 2 /g 100 m 2 /g
125 m 2 /g 130 m 2 /g 100 m 2 /g
-
1.8 µm 2.7 µm
130 m 2 /g
Suraface Area
- -
5 µm
1.8 µm 2.7 µm
7 % 7 % 5 %
9 % 7 % 5 % YES
7 % 7 % 5 % YES
4 % 4 % 3 %
Carbon Load
NA
5 µm
-
End-Cap
No
No
No
pH range
1.0-8.0
2.0-8.0
1.5-8.0
2.0-8.0
2.0-8.0
1.8 µm 2.7 µm
100 MPa*
100 MPa*
100 MPa*
100 MPa*
-
Max Pressure
60 MPa 40 MPa
60 MPa 40 MPa
60 MPa 40 MPa
60 MPa 40 MPa
60 MPa
5 µm
-
* For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 80MPa.
Lot to Lot Reproducibility We understand that lot to lot consistent performance of columns is required to maximize your laboratory performance. From one lot to the next, every Shim-pack Velox column you purchase will perform consistently.
Compounds (50 ng/mL) 1. Cortisol 2. 11-Deoxycortisol 3. Estradiol 4. Boldenone 5. Testosterone 6. Androstenedione 7. Progesterone
Conditions
Column
: Shim-pack Velox Biphenyl 2.7 µm, 3.0×100 mm (P/N: 227-32016-03 )
Flow Rate
: 0.7 mL/min
Column Temp. : 30 °C Sample Dilulent : Initial mobile phase Injection Volume : 5 µL Mobile Phase A
: 0.1 % formic acid in water
Mobile Phase B
: 0.1 % formic acid in acetonitrile Gradient: 40 % B (0 min) 80 % B (3 min)
Pg A-9
Core Shell Columns
Shim-pack Velox SP-C18 Designed and intended specifically for use under low pH condition, Shim-pack Velox SP (Sterically Protected)-C18 offers a well balanced retention profile with a long life time even under harsh, acidic condition needed for LC/MS(/MS) analysis.
Sterically protected to resist strongly acidic (pH 1-8) mobile phase condition Well balanced retention profile Suitable for LC/MS(/MS) analysis
Low pH Stability Sterically protected ligand provides extended low pH stability for the core shell particle. Shim-pack Velox SP-C18 columns maintain a stable retention profile under strongly acidic mobile phase condition (pH1) .
Conditions
Flow Rate
: 0.6 mL/min
Column Temp. Mobile Phase A Mobile Phase B
: 60 °C
: 0.1 % TFA in water
: Acetonitrile
: 45 % B (0 min – 7.5 min) g 5 % B (7.51 min – 50 min) 100 % B (55 min ) g 45 % B (55.01 min g 60 min)
Gradient
Shim-pack Velox SP-C18 Brand A FPP C18 Brand B FPP C18 Brand C FPP C18
Brand D Core-shell C18 Brand E Core-shell C18 Brand F Core-shell C18
Simultaneous Analysis of 22 Preservative Agents More than 20 compounds used as the preservative agent for industrial products like foods and cosmetics can can be separated by Shim-pack Velox SP-C18. The simultaneous determination and quantitation of multiple target compounds are possible in a wide range of commercial product within acceptable analytical times.
Preservative agents 1. 2-methyl-4-Isothiazolin-3-one 2. 2-Bromo-2-Nitro-1,3-propanediol 3. Salicylic Acid 4. Isothiazolinones 5. Benzoic Acid 6. Benzyl Alcohol 7. Sorbic Acid 8. 2-Phenoxyethanol 9. Methyl Paraben 10. Ethyl Paraben 11. Methyl Benzoate
12. Isopropyl Paraben 13. Propyl Paraben 14. 4-Chloro-3-Methylphenol 15. Ethyl Benzoate 16. Isobutyl 4-Hydroxybenzoate
17. Butyl Paraben 18. Chloroxylenol 19. Phenyl Benzoate 20. Clorofene 21. Triclocarban 22. Triclosan
LC Conditions
Column
: Shim-pack Velox SP-C18, 2.7 µm, 3.0×100 mm (PN: 227-32004-03)
Flow Rate
: 1.0 mL/min
Column Temp. Mobile Phase A Mobile Phase B
: 45 °C
: NaH2PO4 aq. (25 mM, pH3.8)
: MeOH/ACN = 9/1
: 8 %B (0 min ) g 30 % B (0.7 min – 2.7 min) 47 % B (2.71 min – 5min ) g 52 % B (6 min) 80 %B (7 min – 8 min) g 8 %B (9.01 min -10 min)
Gradient
Injection Volume : 1 µL Conc.
: 50 mg/L
Pg A-10
Shim-pack Velox SP-C18 Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.
General purpose column for reversed-phase chromatography Highest hydrophobic retention among Shim-pack Velox series Compatible with moderately acidic to neutral mobile phases (pH 2-8)
Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 µm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 µm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.
USP requirement of Chromatography <621> When the column size is changed, the following conditions should be met; 1) L/dp ratio : within -25 % to +50 % or
Number of theoretical plates (N): within -25 % to +50 % (For SPP)
2) Flow rate : *Based on particle size and internal diameter and ±50% L : Column length dp : Particle size F : Flow rate dc : Internal diameter of the column * F 2 = F 1 × dc 2 2 × dp 1 dc 1 2 × dp 2
Original Method
Column
: Shim-pack VP-ODS (5 µm, 4.6×150 mm)
Flow rate
: 0.5 mL/min
Injection Volume : 100 µL Column temp. : 25 °C Detection
: UV 361 nm
Faster Method
Column
: Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate
: 0.50 mL/min
Injection Volume : 67 µL Column temp. : 25 °C Detection
: UV 361 nm
Optimized Method Column
: Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate
: 0.93 mL/min
Injection Volume : 67 µL Column temp. : 25 °C Detection
: UV 361 nm
System suitability test result (Requirement: %RSD < 2.0 %) tR: 0.025 % Area: 0.175 % (n=6) tR: 0.035 % Area: 0.103 % (n=6) tR: 0.084 % Area: 0.220 % (n=6)
Column
L/dp
Flow rate (mL/min)
N
VP-ODS (5 µm, 4.6×150 mm)
30,000
0.50
5,244
9,497 (+81 %) 4,466 (-15 %)
0.50
Velox C18 (2.7 µm, 4.6×100 mm)
37,037 (+23 %)
0.93
Pg A-11
Core Shell Columns
Shim-pack Velox Biphenyl Shim-pack Velox Biphenyl provides enhanced retention of aromatic compounds. It is useful for fast separations in bioanalytical applications due to the increased retention of early eluting analytes such as dipolar, unsaturated and conjugated analytes.
Complementary selectivity to alkyl phases Enhanced separation of aromatic compounds Ideal for increasing sensitivity and selectivity in LC/MS(/MS) analysis
Separating the Structural Isomers Even under the condition where 56 drugs of abuse and metabolites in human urine are quantitated within 10 minutes, two structural isomers, amobarmital and pentobarbital, which have been historically difficult to separate due to their similarity in chemical structures, could be relatively well resolved with shim-pack Velox Biphenyl column.
Amobarbital 5-ethyl-5-(3-methylbutyl)- 1,3-diazinane-2,4,6-trione
Pentobarbital 5-ethyl-5-pentan-2-yl-1,3- diazinane-2,4,6-trione
Conditions
System Column
: Nexera UHPLC System / LCMS-8050 : Shim-pack Velox Biphenyl 2.7 µm, 2.1 mm × 100 mm (PN: 227-32015-03)
Column Temp.
: 30 °C
Flow Rate
: 0.5 mL/min
Mobile Phase A Mobile Phase B
: 0.15 mM ammonium fluoride in water
: Methanol
Gradient : 20 % B (0 min) g 48 % B (1.5 min) g 53 % B (4 min) 100 %B (6 min - 7.5 min) g 20 % B (7.51 min - 9.5 min) Sample preparation : Enzymatically hydrolyzed human urine
was spiked with target compounds between 10 - 1000 % of the required cut off concentration. Samples were subsequently diluted 5x with 0.1 % formic acid.
Shim-pack Velox PFPP Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to C18 columns and is suitable for the analysis of halogenated compounds, positional isomers and charged bases.
Alternative selectivity to C18 columns Suitable for positional isomers and halogenated compounds Offers increased retention for charged bases
Good Separation of Cis / Trans Stilbene Cis and trans isomers of stilbene that are difficult to resolve with an ODS column due to their similarity in hydrophobicity can be well separated with Shim-pack Velox PFPP column.
1. cis-Stilbene
2. trans-Stilbene
LC Conditions
Column
: Shim-pack Velox PFPP, 2.7 µm, 3.0×100 mm (PN: 227-32022-04)
Column Temp.
: 40 °C
Flow Rate
: 0.4 mL/min
Mobile Phase
: Methanol/Water = 9/1
Detection
: UV 254 nm
Sample
: 1. cis-Stilbene, 2. trans-Stilbene
Injection Volume
: 1 µL
Pg A-12
Shim-pack Velox HILIC Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. Shim-pack Velox HILIC using unbonded core shell particles is specifically designed for this application.
Orthogonal selectivity to reversed phase chromatography Increased retention of polar analytes Increased MS sensitivity Direct compatibility with sample preparation eluates
Retention of Nucleosides Nucleosides are polar molecules that are not well retained on reversed phase LC columns due to their hydrophilic nature are well retained and separated with Shim-pack Velox HILIC column.
1. Toluene 2. Uracil 3. Uridine 4. Adenosine 5. Guanosine 6. Cytidine
LC Conditions
Column
: Shim-pack Velox HILIC, 2.7 µm, 3.0×100 mm (PN: 227-32026-02)
Column Temp.
: 30 ºC
Flow Rate
: 0.4 mL/min
Mobile Phase
: Acetonitrile/20mM AcONH4aq.=9/1
Detection
: UV 254 nm
Sample
: 1. Toluene, 2. Uracil, 3. Uridine, 4. Adenosine, 5. Guanosine, 6. Cytidine
Injection Volume
: 1 µL
Shim-pack Velox 1.8 µm Columns
Chemistry
SP-C18
C18
Biphenyl
PFPP
I.D. (mm)
2.1
3.0
2.1
3.0
2.1
3.0
2.1
3.0
Length (mm)
30
227-32001-01
-
227-32007-01
-
227-32013-01
-
227-32019-01
-
50 227-32001-02 227-32002-01 227-32007-02 227-32008-01 227-32013-02 227-32014-01 227-32019-02 227-32020-01 100 227-32001-03 227-32002-02 227-32007-03 227-32008-02 227-32013-03 227-32014-02 227-32019-03 227-32020-02 150 227-32001-04 - 227-32007-04 - 227-32013-04 - 227-32019-04 -
Shim-pack Velox 2.7 µm Columns
Chemistry
SP-C18
C18
Biphenyl
I.D. (mm)
2.1
3.0
4.6
2.1
3.0
4.6
2.1
3.0
4.6
Length (mm)
30 227-32003-01 227-32004-01 227-32005-01 227-32009-01 227-32010-01 227-32011-01 227-32015-01 227-32016-01 227-32017-01 50 227-32003-02 227-32004-02 227-32005-02 227-32009-02 227-32010-02 227-32011-02 227-32015-02 227-32016-02 227-32017-02 100 227-32003-03 227-32004-03 227-32005-03 227-32009-03 227-32010-03 227-32011-03 227-32015-03 227-32016-03 227-32017-03 150 227-32003-04 227-32004-04 227-32005-04 227-32009-04 227-32010-04 227-32011-04 227-32015-04 227-32016-04 227-32017-04
Chemistry
PFPP
HILIC
I.D. (mm)
2.1
3.0
4.6
2.1
3.0
4.6
Length (mm)
30 50
227-32021-01 227-32022-01 227-32023-01 227-32025-01
-
-
227-32021-02 227-32022-02 227-32023-02 227-32025-02 227-32026-01 227-32027-01 227-32021-03 227-32022-03 227-32023-03 227-32025-03 227-32026-02 227-32027-02 227-32021-04 227-32022-04 227-32023-04 227-32025-04 227-32026-03 227-32027-03
100 150
Pg A-13
Core Shell Columns
Shim-pack Velox 5 µm Columns
Chemistry
SP-C18
C18
Biphenyl
PFPP
I.D. (mm)
4.6
Length (mm)
30 50
227-32006-01 227-32012-01 227-32018-01 227-32024-01 227-32006-02 227-32012-02 227-32018-02 227-32024-02 227-32006-03 227-32012-03 227-32018-03 227-32024-03 227-32006-04 227-32012-04 227-32018-04 227-32024-04
100 150
Shim-pack Velox EXP Guard Column Cartridge (3 pk)
Type
1.8 µm
2.7 µm
I.D. x L (mm)
SP-C18
C18
Biphenyl
PFPP
SP-C18
C18
Biphenyl
PFPP
HILIC
2.1 x 5 227-32028-01 227-32031-01 227-32034-01 227-32037-01 227-32029-01 227-32032-01 227-32035-01 227-32038-01 227-32040-01 3.0 x 5 227-32028-02 227-32031-02 227-32034-02 227-32037-02 227-32029-02 227-32032-02 227-32035-02 227-32038-02 227-32040-02 4.6 x 5 - - - - 227-32029-03 227-32032-03 227-32035-03 227-32038-03 227-32040-03
Type
5 μm
I.D. x L (mm)
SP-C18
C18
Biphenyl
PFPP
4.6 x 5
227-32030-01 227-32033-01 227-32036-01 227-32039-01
*Shim-pack Velox EXP Direct Connect Holder : 227-32041-01
Shim-pack Velox UHPLC Pre-column (0.2 μm)
1pc
227-32042-01
5pcs
227-32042-02
10pcs 227-32042-03 *For more pre-column options, please refer to page 110
Shim-pack Velox EXP Guard Column Free-turning architecture lets you change cartridges by hand without breaking inlet / outlet fluid connections — no tools needed. Guard column cartridges require Shim-pack Velox EXP Direct Connect Holder (227-32041-01)
Shim-pack Velox UHPLC Precolumn Filter (0.2 µm) To minimize extra column volume and maximize UHPLC sample throughput with SPE, SLE, or other sample preparation techniques, pair 1.8 µm Shim-pack Velox UHPLC columns with an Shim-pack Velox UHPLC Precolumn filter instead of a guard cartridge.
Pg A-14
UHPLC/HPLC Columns
Shim-pack Scepter LC Columns
Excellent stability & performance could be achieved under a wide range of LC conditions Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 µm, 3 µm, 5 µm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.
Shim-pack Scepter Chemistries
Reversed Phase
HILIC
Shim-pack Scepter
C18
HD-C18
C8
Pheny
PFPP
C4
Diol-HILIC
Trifunctional C18 Trifunctional C18
Trifunctional Phenylbutyl
Trifunctional Pentafluorophenylpropyl
Trifunctional Dihydroxypropyl
Ligand Type
Trifunctional C8
Butyl group
Generic Purpose Type
High Density Type
Particle
Organic Silica Hybrid
Particle Size
1.9 μm, 3 μm, 5 μm
Pore Size
12 nm
8 nm
12 nm
30 nm
12 nm
End Capping
Proprietary
None
Proprietary
None
pH Range
1 - 12
1 - 10
1 - 8
1 - 10
2 - 10
100% aqueous condition
Yes
No
No
Yes
Yes
No
N/A
USP Classification
L1
L1
L7
L11
L43
L26
L20
Method Scouting Utilize excellent stability & performance under a wide range of LC conditions
With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.
Comparison of Chromatograms using Gradient condition with Acetonitrile
Analytes 1. Saccharin (pKa = 2.2) 2. Dextromethorphan (pKa = 8.3) 3. Amitriptyline (pKa = 9.4) 4. N-Butyl paraben (pKa = 8.3) 5. Ibupurofen (pKa = 4.4)
For Shim-pack Scepter C4, please refer to page A-22
Pg A-15
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