Column Selection Guide
Column Selection by Target Compounds
Shim-pack Velox C18/SP-C18......A-10 Shim-pack Scepter......................A-16 Shim-pack GIST C18....................A-38
Mixture of Water-soluble / Fat-soluble vitamins
Reversed Phase
Vitamin C
Shim-pack GIST NH2...................A-60
HILIC Mode
Water-soluble Vitamins
Shim-pack Scepter C18...............A-16 Shim-pack MAqC-ODS................A-36
Reversed Phase
Vitamins
Others
HILIC Mode
Shim-pack Scepter Dio-HILIC......A-21
Shim-pack Velox..........................A-10 Shim-pack Scepter C18...............A-16 Shim-pack GIST C18....................A-38
Vitamin A, K
Reversed Phase
Fat-soluble Vitamins
Shim-pack GIST NH2...................A-60
Vitamin D, E
Normal Phase
Fat-soluble Vitamins
Water-soluble Vitamins
UV (325 nm) RF (Ex. 340 nm, Em. 460 nm)
UV (270 nm) RF (Post-column derivatization) UV (270 nm) RF (Post-column derivatization)
Vitamin A (retinol)
Vitamin B1 (thiamin)
Vitamin D
UV (265 nm)
Vitamin B2 (riboflavin)
UV (295 nm) RF (Ex. 295 nm, Em. 325 nm) UV (250 nm) RF (Post-column derivatization)
Vitamin E (tocopherol)
Vitamin B3 (niacin)
UV (260 nm) UV (205 nm) UV (290 nm) UV (280 nm) UV (210 nm) RI UV (245 nm) UV (210 nm) UV (280 nm) UV (265 nm)
Vitamin B5 (pantothenic acid)
Vitamin K
Vitamin B6 (pyridoxine)
Vitamin B12 (cyanocobalamine)
Vitamin BT (carnitine)
Vitamin C (ascorbic acid)
Vitamin H (biotin)
Vitamin M (folic acid) Vitamin P (hesperidin)
* The above wavelength values are approximate values. It is possible to be influenced by the state of mobile phase.
Shim-pack Bio IEX SP-NP/SP........A-77 Shim-pack WCX-1.......................A-99
Cation Exchange
Shim-pack Velox C18/SP-C18......A-10 Shim-pack Scepter C18/HD-C18..A-16 Shim-pack GIST C18....................A-38 Shim-pack Velox HILIC................A-13 Shim-pack Scepter Diol HILIC.....A-21 Shim-pack GIS HILIC....................A-58
Reversed Phase
Nucleic Acid Bases and Nucleosides
HILIC Mode
Shim-pack Bio IEX Q/Q-NP..........A-77 Shim-pack WAX-1.......................A-99
Anion Exchange
Nucleic Acids
Nucleotides
Shim-pack Velox..........................A-10 Shim-pack Scepter C18...............A-16 Shim-pack GIST C18....................A-38
Reversed Phase
Gel Filtration
Shim-pack Bio Diol......................A-77 Shim-pack Diol Series.................A-85
DNA and RNA
Shim-pack Bio IEX Q/Q-NP..........A-77 Shim-pack WAX-2.......................A-99
Anion Exchange
Pg A-6
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