Analysis of Nucleotides, Oligonucleotides and Protein
Shim-pack WAX/WCX Series Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. Shim-pack WAX-1 is ideal for analysis of nucleotides and oligonucleotides while Shim-pack WAX-2 and WCX-1 is ideal for analysis of proteins.
Peaks 1. UMP 7. ADP 2. CMP 8. UTP 3. AMP 9. GDP 4. GMP 10. CTP 5. UDP 11. ATP 6. CDP 12. GTP
Conditions
Column
: Shim-pack WAX-1 (50 mmL × 4.0 mmI.D., 3 μm) (P/N: 228-16225-91)
Mobile Phase : A) 20mM phosphate buffer solution (pH 7)
B) 480mM phosphoric acid buffer solution (pH 6.85) gradient elution
Flow Rate Col. Temp. Detection
: 1.0 mL/min
: 45 °C
: UV 260 nm
Analysis of Mononucleotides
Peaks 1. Ovalbumin 2. Myoglobin 3. α -Chymotrypsinogen A 4. Ribonuclease A 5. Lysozyme
Conditions
Column
: Shim-pack WCX-1 (50 mmL × 4.0 mmI.D., 5 μm) (P/N: 228-16366-91)
Mobile Phase : A) 20mM phosphate buffer solution (pH 6.0) B) Sodium sulfate gradient elution Flow Rate : 1.0 mL/min Col. Temp. : Ambient Detection : UV 415 nm
Analysis of Protein Standard
Product Information
Particle Size (μm)
Dimensions (Length × I.D., mm)
Column
Stationary Phase
P/N
Shim-pack WAX-1 Shim-pack WAX-1 Shim-pack WAX-1T Shim-pack WAX-2 Shim-pack WCX-1 Pre-column Diol *
Tertiary amino group Tertiary amino group Tertiary amino group Tertiary amino group
3 3 3 5 5
50 × 4.0
228-16225-91 228-16225-92 228-18257-91 228-16365-91 228-16366-91 228-16367-91
150 × 4.6 50 × 4.6 50 × 4.0 50 × 4.0 50 × 4.0
Carboxyl group
Diol group
10
* Installed between the liquid pump and the sample injector to protect the column.
Pg A-99
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