Pretreatment Columns
Shim-pack MAYI Series Due to optimized particle size and a newly developed coating technology, the MAYI series online pretreatment column is highly effective in deproteinization and offers long-term stability. It provides excellent reproducibility even for continuous analysis of multiple analytes.
How the Shim-pack MAYI Series Works The outer surfaces of silica gel (50 μm) are coated with a hydrophilic polymer, so that only the interior of pores are chemically modified by octadecyl radicals (ODS). Since proteins and other macromolecules cannot enter the pores and are blocked by the hydrophilic polymer on the outer surfaces, they are quickly eluted without being retained by the ODS solid phase. In contrast, pharmaceuticals and other induced low molecular weight compounds penetrate the pores and are retained by the inner surfaces of the stationary phase.
Quick and Reliable Protein Removal The newly developed hydrophilic polymer coating technology quickly and reliably removes macromolecules, such as protein, from injected biological samples to achieve high recovery rates for target components. In addition to securely protecting analytical columns and LC/MS interfaces, this also helps reduce the time required for finishing the analysis. Outstanding Durability Due to the polymer coating technology and particle size optimization, stable data can be obtained for long periods. The figure below shows results from 300 consecutive injections of 100 μL of blood plasma. No decrease in the deproteinization rate or degradation of peak shape was observed.
Comparison of 1st and 300th Analyses
Conditions
Samples
: Isopropylantipyrine added Blood plasma Sample solution: 0.1% phosphoric acid and acetonitrile mixture (95:5) Dilution: 8 times
Detection
: Analysis: 275 nm, Blood plasma matrix: 280 nm
Injection Vol. : 100 μL
Pg A-106
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