Stable Trap Even for Large Injection Volumes The Shim-pack MAYI-ODS column provides stable component recovery rates and protein removal, even when injecting large volumes. Even when 500 μL of blood plasma was directly injected, a high recovery rate was obtained and no peak distortion was observed.
Conditions
Samples
: Indomethacin added Blood plasma Sample solution: 0.1% phosphoric acid and acetonitrile mixture (95:5) Dilution: 8 times
Detection
: UV 315 nm
Comparison Data for Injecting 100, 200, and 500 μL
Product Information
Column
Stationary Phase
Separation Mode
Shim-pack MAYI-ODS(G) Shim-pack MAYI-C14(G) Shim-pack MAYI-C8(G) Shim-pack MAYI-C4(G) Shim-pack MAYI-C1(G) Shim-pack MAYI-SCX(G) Shim-pack MAYI-SAX(G)
Octadecyl group
Strongest retentivity in reversed phase mode
Tetradecyl group
Retentivity next to ODS in reversed phase mode Retentivity next to C14 in reversed phase mode
Octyl group Butyl group Methyl group
Retentivity next to C8 in reversed phase mode
Weakest retentivity in reversed phase mode
Sulfonic acid group
Strong acid cation exchange mode
Trimethylammonium group
Weakly basic anion exchange mode
Cartridge
Column Holder
Particle Size (μm)
Dimensions (Length × I.D., mm)
Dimensions (Length × I.D., mm)
Column
P/N
P/N
5 × 2.0
228-40835-93 228-40835-95 228-40835-91 228-40835-97 228-46185-91 228-46186-91 228-46187-91 228-46188-91 228-45366-91 228-45366-93 228-45370-91 228-45370-93
5 × 2.0
228-34938-94 228-34938-98 228-34938-92 228-34938-96
10 × 2.0 10 × 4.6 30 × 4.6 10 × 4.6 10 × 4.6 10 × 4.6 10 × 4.6 10 × 4.6 30 × 4.6 10 × 4.6 30 × 4.6
10 × 2.0 10 × 4.6 30 × 4.6
Shim-pack MAYI-ODS
50
Shim-pack MAYI-C1 Shim-pack MAYI-C4 Shim-pack MAYI-C8 Shim-pack MAYI-C14
50 50 50 50
Shim-pack MAYI-SAX
50
Shim-pack MAYI-SCX
50
Pg A-107
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