Column Chemistries Combining highly efficient core shell particle technology with a wide range of surface chemistries provides you with the best opportunity for optimal resolution. With different chemistry characteristics, Shim-pack Velox columns are suitable for use in a wide variety of applications and challenging separations.
SP-C18
C18
Biphenyl
PFPP
HILIC
USP Classification
L1
L1
L11
L43
L3
Sterically protected C18
Pentafluorophenyl propyl
Ligand type
C18
Biphenyl
None
Particle Size (µm)
1.8, 2.7, 5
1.8, 2.7, 5
1.8, 2.7, 5
1.8, 2.7, 5
2.7
Pore size (Å)
90
90
90
90
90
125 m 2 /g 130 m 2 /g 100 m 2 /g
125 m 2 /g 130 m 2 /g 100 m 2 /g
125 m 2 /g 130 m 2 /g 100 m 2 /g
125 m 2 /g 130 m 2 /g 100 m 2 /g
-
1.8 µm 2.7 µm
130 m 2 /g
Suraface Area
- -
5 µm
1.8 µm 2.7 µm
7 % 7 % 5 %
9 % 7 % 5 % YES
7 % 7 % 5 % YES
4 % 4 % 3 %
Carbon Load
NA
5 µm
-
End-Cap
No
No
No
pH range
1.0-8.0
2.0-8.0
1.5-8.0
2.0-8.0
2.0-8.0
1.8 µm 2.7 µm
100 MPa*
100 MPa*
100 MPa*
100 MPa*
-
Max Pressure
60 MPa 40 MPa
60 MPa 40 MPa
60 MPa 40 MPa
60 MPa 40 MPa
60 MPa
5 µm
-
* For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 80MPa.
Lot to Lot Reproducibility We understand that lot to lot consistent performance of columns is required to maximize your laboratory performance. From one lot to the next, every Shim-pack Velox column you purchase will perform consistently.
Compounds (50 ng/mL) 1. Cortisol 2. 11-Deoxycortisol 3. Estradiol 4. Boldenone 5. Testosterone 6. Androstenedione 7. Progesterone
Conditions
Column
: Shim-pack Velox Biphenyl 2.7 µm, 3.0×100 mm (P/N: 227-32016-03 )
Flow Rate
: 0.7 mL/min
Column Temp. : 30 °C Sample Dilulent : Initial mobile phase Injection Volume : 5 µL Mobile Phase A
: 0.1 % formic acid in water
Mobile Phase B
: 0.1 % formic acid in acetonitrile Gradient: 40 % B (0 min) 80 % B (3 min)
Pg A-9
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