Core Shell Columns
Shim-pack Velox SP-C18 Designed and intended specifically for use under low pH condition, Shim-pack Velox SP (Sterically Protected)-C18 offers a well balanced retention profile with a long life time even under harsh, acidic condition needed for LC/MS(/MS) analysis.
Sterically protected to resist strongly acidic (pH 1-8) mobile phase condition Well balanced retention profile Suitable for LC/MS(/MS) analysis
Low pH Stability Sterically protected ligand provides extended low pH stability for the core shell particle. Shim-pack Velox SP-C18 columns maintain a stable retention profile under strongly acidic mobile phase condition (pH1) .
Conditions
Flow Rate
: 0.6 mL/min
Column Temp. Mobile Phase A Mobile Phase B
: 60 °C
: 0.1 % TFA in water
: Acetonitrile
: 45 % B (0 min – 7.5 min) g 5 % B (7.51 min – 50 min) 100 % B (55 min ) g 45 % B (55.01 min g 60 min)
Gradient
Shim-pack Velox SP-C18 Brand A FPP C18 Brand B FPP C18 Brand C FPP C18
Brand D Core-shell C18 Brand E Core-shell C18 Brand F Core-shell C18
Simultaneous Analysis of 22 Preservative Agents More than 20 compounds used as the preservative agent for industrial products like foods and cosmetics can can be separated by Shim-pack Velox SP-C18. The simultaneous determination and quantitation of multiple target compounds are possible in a wide range of commercial product within acceptable analytical times.
Preservative agents 1. 2-methyl-4-Isothiazolin-3-one 2. 2-Bromo-2-Nitro-1,3-propanediol 3. Salicylic Acid 4. Isothiazolinones 5. Benzoic Acid 6. Benzyl Alcohol 7. Sorbic Acid 8. 2-Phenoxyethanol 9. Methyl Paraben 10. Ethyl Paraben 11. Methyl Benzoate
12. Isopropyl Paraben 13. Propyl Paraben 14. 4-Chloro-3-Methylphenol 15. Ethyl Benzoate 16. Isobutyl 4-Hydroxybenzoate
17. Butyl Paraben 18. Chloroxylenol 19. Phenyl Benzoate 20. Clorofene 21. Triclocarban 22. Triclosan
LC Conditions
Column
: Shim-pack Velox SP-C18, 2.7 µm, 3.0×100 mm (PN: 227-32004-03)
Flow Rate
: 1.0 mL/min
Column Temp. Mobile Phase A Mobile Phase B
: 45 °C
: NaH2PO4 aq. (25 mM, pH3.8)
: MeOH/ACN = 9/1
: 8 %B (0 min ) g 30 % B (0.7 min – 2.7 min) 47 % B (2.71 min – 5min ) g 52 % B (6 min) 80 %B (7 min – 8 min) g 8 %B (9.01 min -10 min)
Gradient
Injection Volume : 1 µL Conc.
: 50 mg/L
Pg A-10
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