Shim-pack Velox SP-C18 Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.
General purpose column for reversed-phase chromatography Highest hydrophobic retention among Shim-pack Velox series Compatible with moderately acidic to neutral mobile phases (pH 2-8)
Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 µm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 µm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.
USP requirement of Chromatography <621> When the column size is changed, the following conditions should be met; 1) L/dp ratio : within -25 % to +50 % or
Number of theoretical plates (N): within -25 % to +50 % (For SPP)
2) Flow rate : *Based on particle size and internal diameter and ±50% L : Column length dp : Particle size F : Flow rate dc : Internal diameter of the column * F 2 = F 1 × dc 2 2 × dp 1 dc 1 2 × dp 2
Original Method
Column
: Shim-pack VP-ODS (5 µm, 4.6×150 mm)
Flow rate
: 0.5 mL/min
Injection Volume : 100 µL Column temp. : 25 °C Detection
: UV 361 nm
Faster Method
Column
: Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate
: 0.50 mL/min
Injection Volume : 67 µL Column temp. : 25 °C Detection
: UV 361 nm
Optimized Method Column
: Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate
: 0.93 mL/min
Injection Volume : 67 µL Column temp. : 25 °C Detection
: UV 361 nm
System suitability test result (Requirement: %RSD < 2.0 %) tR: 0.025 % Area: 0.175 % (n=6) tR: 0.035 % Area: 0.103 % (n=6) tR: 0.084 % Area: 0.220 % (n=6)
Column
L/dp
Flow rate (mL/min)
N
VP-ODS (5 µm, 4.6×150 mm)
30,000
0.50
5,244
9,497 (+81 %) 4,466 (-15 %)
0.50
Velox C18 (2.7 µm, 4.6×100 mm)
37,037 (+23 %)
0.93
Pg A-11
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