Core Shell Columns
Shim-pack Velox Biphenyl Shim-pack Velox Biphenyl provides enhanced retention of aromatic compounds. It is useful for fast separations in bioanalytical applications due to the increased retention of early eluting analytes such as dipolar, unsaturated and conjugated analytes.
Complementary selectivity to alkyl phases Enhanced separation of aromatic compounds Ideal for increasing sensitivity and selectivity in LC/MS(/MS) analysis
Separating the Structural Isomers Even under the condition where 56 drugs of abuse and metabolites in human urine are quantitated within 10 minutes, two structural isomers, amobarmital and pentobarbital, which have been historically difficult to separate due to their similarity in chemical structures, could be relatively well resolved with shim-pack Velox Biphenyl column.
Amobarbital 5-ethyl-5-(3-methylbutyl)- 1,3-diazinane-2,4,6-trione
Pentobarbital 5-ethyl-5-pentan-2-yl-1,3- diazinane-2,4,6-trione
Conditions
System Column
: Nexera UHPLC System / LCMS-8050 : Shim-pack Velox Biphenyl 2.7 µm, 2.1 mm × 100 mm (PN: 227-32015-03)
Column Temp.
: 30 °C
Flow Rate
: 0.5 mL/min
Mobile Phase A Mobile Phase B
: 0.15 mM ammonium fluoride in water
: Methanol
Gradient : 20 % B (0 min) g 48 % B (1.5 min) g 53 % B (4 min) 100 %B (6 min - 7.5 min) g 20 % B (7.51 min - 9.5 min) Sample preparation : Enzymatically hydrolyzed human urine
was spiked with target compounds between 10 - 1000 % of the required cut off concentration. Samples were subsequently diluted 5x with 0.1 % formic acid.
Shim-pack Velox PFPP Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to C18 columns and is suitable for the analysis of halogenated compounds, positional isomers and charged bases.
Alternative selectivity to C18 columns Suitable for positional isomers and halogenated compounds Offers increased retention for charged bases
Good Separation of Cis / Trans Stilbene Cis and trans isomers of stilbene that are difficult to resolve with an ODS column due to their similarity in hydrophobicity can be well separated with Shim-pack Velox PFPP column.
1. cis-Stilbene
2. trans-Stilbene
LC Conditions
Column
: Shim-pack Velox PFPP, 2.7 µm, 3.0×100 mm (PN: 227-32022-04)
Column Temp.
: 40 °C
Flow Rate
: 0.4 mL/min
Mobile Phase
: Methanol/Water = 9/1
Detection
: UV 254 nm
Sample
: 1. cis-Stilbene, 2. trans-Stilbene
Injection Volume
: 1 µL
Pg A-12
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