Shim-pack Velox HILIC Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. Shim-pack Velox HILIC using unbonded core shell particles is specifically designed for this application.
Orthogonal selectivity to reversed phase chromatography Increased retention of polar analytes Increased MS sensitivity Direct compatibility with sample preparation eluates
Retention of Nucleosides Nucleosides are polar molecules that are not well retained on reversed phase LC columns due to their hydrophilic nature are well retained and separated with Shim-pack Velox HILIC column.
1. Toluene 2. Uracil 3. Uridine 4. Adenosine 5. Guanosine 6. Cytidine
LC Conditions
Column
: Shim-pack Velox HILIC, 2.7 µm, 3.0×100 mm (PN: 227-32026-02)
Column Temp.
: 30 ºC
Flow Rate
: 0.4 mL/min
Mobile Phase
: Acetonitrile/20mM AcONH4aq.=9/1
Detection
: UV 254 nm
Sample
: 1. Toluene, 2. Uracil, 3. Uridine, 4. Adenosine, 5. Guanosine, 6. Cytidine
Injection Volume
: 1 µL
Shim-pack Velox 1.8 µm Columns
Chemistry
SP-C18
C18
Biphenyl
PFPP
I.D. (mm)
2.1
3.0
2.1
3.0
2.1
3.0
2.1
3.0
Length (mm)
30
227-32001-01
-
227-32007-01
-
227-32013-01
-
227-32019-01
-
50 227-32001-02 227-32002-01 227-32007-02 227-32008-01 227-32013-02 227-32014-01 227-32019-02 227-32020-01 100 227-32001-03 227-32002-02 227-32007-03 227-32008-02 227-32013-03 227-32014-02 227-32019-03 227-32020-02 150 227-32001-04 - 227-32007-04 - 227-32013-04 - 227-32019-04 -
Shim-pack Velox 2.7 µm Columns
Chemistry
SP-C18
C18
Biphenyl
I.D. (mm)
2.1
3.0
4.6
2.1
3.0
4.6
2.1
3.0
4.6
Length (mm)
30 227-32003-01 227-32004-01 227-32005-01 227-32009-01 227-32010-01 227-32011-01 227-32015-01 227-32016-01 227-32017-01 50 227-32003-02 227-32004-02 227-32005-02 227-32009-02 227-32010-02 227-32011-02 227-32015-02 227-32016-02 227-32017-02 100 227-32003-03 227-32004-03 227-32005-03 227-32009-03 227-32010-03 227-32011-03 227-32015-03 227-32016-03 227-32017-03 150 227-32003-04 227-32004-04 227-32005-04 227-32009-04 227-32010-04 227-32011-04 227-32015-04 227-32016-04 227-32017-04
Chemistry
PFPP
HILIC
I.D. (mm)
2.1
3.0
4.6
2.1
3.0
4.6
Length (mm)
30 50
227-32021-01 227-32022-01 227-32023-01 227-32025-01
-
-
227-32021-02 227-32022-02 227-32023-02 227-32025-02 227-32026-01 227-32027-01 227-32021-03 227-32022-03 227-32023-03 227-32025-03 227-32026-02 227-32027-02 227-32021-04 227-32022-04 227-32023-04 227-32025-04 227-32026-03 227-32027-03
100 150
Pg A-13
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