Appendix I
Syringe filter order guideline Choosing the right membrane is important. The wrong membrane could cause loss of valuable samples, time and money. Please use below guideline and chemical compatibility table as reference.
• Naturally hydrophilic, high protein binding, high dirt-loading, surfactant-free and offer the lowest extractables. • Filtering of general samples and organic solvents. • Not recommended for use with acids. • Extremely low protein binding. • Good chemical compatibility. • For filtration of non-aggressive aqueous and mild organic solutions, or when maximizing protein recovery is important.
Hydrophilic Nylon
pH 6-13
Hydrophilic PVDF (polyvinylidene fluoride)
pH 3-12
• Hydrophilic membrane with good solvent resistance. • Low protein binding and extractables.
Hydrophilic RC (regenerated cellulose)
pH 3-12
• Good chemical compatibility with nearly all common HPLC solvents and stable against DMSO. • Good for general filtration, tissue culture media filtration or life science applications.
Hydrophilic PES (polyethersulfone)
• Low protein binding and extractables. • Good for life science applications.
pH 3-12
• High chemical compatibility. • High temperature resistance. • Good for filtration of gases, aggressive chemicals, and strong acid.
PTFE
pH 1-14
• Low protein binding • For filtration of aqueous solutions • Good for applications involving culture media • High protein binding • Good for general filtration of aqueous solutions.
Cellulose Acetate (CA)
pH 4-8
Mixed Cellulose Ester (MCE)
pH 4-8
Solvent Compatibility:
Solvent
MCE
CA
PES
Nylon
PVDF
PTFE
RC
Acetic Acid (glacial)
X
X
O
X
O
O
LR
Acetone
X
X
X
O
X
O
O
Acetonitrile (ACN)
X
X
X
O
LR
O
O
Benzene
O
O
X
O
O
O
O
Butyl Alcohol
O
O
O
O
O
O
O
Chloroform
O
X
X
X
O
O
O
Cyclohexanone
X
X
X
O
X
O
O
Dimethyl Sulfoxide (DMSO)
X
X
X
O
X
O
O
Ethyl Acetate
X
X
X
O
O
O
O
Ethyl Alcohol
X
O
O
O
LR
O
O
Formaldehyde
X
X
NI
O
O
O
LR
Hexane
O
O
O
O
O
O
O
Hydochloric Acid, 1N (HCl)
LR
LR
O
LR
O
O
X
Hydochloric Acid, 6N (HCl)
LR
X
LR
X
LR
O
X
Hydochloric Acid, 12N (HCl)
X
X
LR
X
X
O
X
Hydrogen Peroxide, 3%
X
O
NI
O
O
O
X
Isopropyl Alcohol
X
O
O
LR
O
O
O
Methanol
X
O
O
LR
O
O
O
Nitric Acid, 6N (HNO3)
X
O
O
X
O
O
X
Nitric Acid, 12N (HNO3)
X
X
NI
X
X
LR
X
Sodium Carbonate (aqueous)
O
NI
NI
LR
O
O
NI
Sodium Hydroxide, 3N (NaOH)
X
X
O
O
O
O
X
Sulfuric Acid, 6N (H2SO4)
LR
X
LR
X
LR
O
LR
Sulfuric Acid, 32N (H2SO4)
X
X
X
X
LR
LR
X
Tetrahydrofuran (THF)
X
X
NI
O
X
O
O
Toluene
O
O
O
O
O
O
O
O: Recommended
LR: Limited recommendation
X: Not recommended
NI: No info
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