Shim-pack Arata LC Columns Unprecedented Resolution and Peak Shape of Basic Compounds
Even for LC columns that claim to be designed for basic compounds, adequate resolution often can not be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for basic compounds. Unmatched Peak Shape (Elegantly Designed for A Wide Range of Compounds) Unmatched peak shape of basic compounds could be achieved while maintaining good peak shape for acidic compounds with Shim-pack Arata LC columns. Even with low ionic strength acidic mobile phase, such as 0.1% formic acid containing mobile phase, excellent peak shape of both amitriptyline (a basic compound) and benzoic acid (an acidic compound) could be achieved.
Analytical Conditions Column
: Shim-pack Arata C18
Mobile Phase
: 0.1% HCOOH in H2O / CH3CN = 70 / 30
Flow Rate Detection
: 0.4 mL/min
: 254 nm
Column Temp.
: 40 °C : 2 μL
Inj. Volume
* Gel Lot QC test analytical condition
Excellent Separation Performance for Peptides (Even with Weak Ion Paring Acids) In order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS (/MS) analysis. Excellent peak shape and separation performance for peptides could be achieved on the Shim-pack Arata LC column even with 0.1 % formic acid (weak ion paring acid) containing mobile phase.
Analytical Conditions Column
: Shim-pack Arata C18
Mobile Phase
: A: 0.1% HCOOH in H2O : B: 0.1% HCOOH in CH3CN
Flow Rate Detection
: 0.4 mL/min
: 214 nm
Column Temp.
: 40 °C : 1 μL
Inj. Volume
Sample
: Angiotensin
Vial
: TORAST-H™ Bio Vial
* Gel Lot QC test analytical condition
Column
Asymmetry
Shim-pack Arata C18 Typical ODS column
1.26 6.94
* Peptide is usually analyzed using gradient condition. Isocratic condition was used for this application in order to show the difference of LC columns more clearly. Result using gradient condition on Shim-pack Arata C18 was also evaluated and it was confirmed that angiotensin was fully eluted from the column with isocratic condition. ** Acetonitrile concentration was adjusted in order that the retention time of peptide on each column become similar.
Pg A-24
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