High Peptide Recovery enabled by Shim-pack Arata technology
Since the adsorption of peptides to particles is minimized, high recovery of peptides is ensured and excellent peptide analysis can be provided by Shim-pack Arata Peptide C18 column.
Analysis of Tryptic Digest of Myoglobin
Peptides are known to show non-specific adsorption to particles of LC columns. A Shim-pack Arata Peptide C18 column showed high recovery of peptides.
Analytical Conditions LC System : NexeraX2MP_M30A (STD Cell) LC Column : Shim-pack Arata Peptide C18 (2.0 × 150 mm, 2.2 μm) Core shell Peptide C18 (2.1×150 mm, sub 2 μm) Core shell C8 (2.1 ×150 mm, sub 4 μm) Mobile Phase A : 0.1% HCOOH in H2O Mobile Phase B : 0.1% HCOOH in CH3CN Gradient : 2%B (0-5 min) 45%B (20 min) 100%B (20.01 – 25 min) 2%B (25.01 – 30 min)
Detection : 214 nm Column temp. : 40 °C Injection volume : 1 μL Sample : Angiotensin I Vial : Torast-H Bio Vial
Tryptic digest of myoglobin was analyzed using Shim-pack Arata Peptide C18 (2.2 μm), Core shell C18 (sub 2 μm) and a Core shell C8 (sub 4 μm). Compared to the other two columns, Shim-pack Arata Peptide C18 showed significantly larger peak area, which suggests that higher recovery could be obtained with Shim-pack Arata Peptide C18 column.
Shim-pack Arata Peptide C18
I.D. (mm)
Particle Size (μm)
2.0
Length (mm)
50
227-32806-01 227-32806-02 227-32806-03
2.2
100 150
Pg A-27
Made with FlippingBook Digital Proposal Creator