Example of the Batch Analysis of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)
Conditions
Column
: Top
: Shim-pack VP-Phenyl (150 mmL. × 4.6 mmI.D., 5 μm) (P/N: 228-59928-91) Bottom : Shim-pack XR-Phenyl (50 mmL. × 3.0 mmI.D., 2.2 μm) (P/N: 228-59904-92)
Mobile Phase : A) 20 mmol/L Phosphoric acid (Na) buffer (pH 2.5) B) Acetonitrile A/B = 3/7 (v/v) Flow Rate : Top : 1.0 mL/min Bottom : 1.0 mL/min
Relative Retention Times
Peak
Shim-pack VP-Phenyl
Shim-pack XR-Phenyl
1. Piroxicam
1.000 1.480 2.233 2.949 3.263 3.470
1.000 1.483 2.172 2.916 3.262 3.501
2. Ketoprofen 3. Diflunisal 4. Diclofenac 5. Indomethacin
6. Ibuprofen
Columns with the same size but different bonded phases are available as a set for the purpose of method development. Please contact your local Shimadzu representative for details.
Check out the Nexera Method Scouting System at www.shimadzu.com/an/hplc
Pg A-35
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