UHPLC/HPLC Columns
Shim-pack GISS C18
Ideal for Rapid Analysis Shim-pack GISS C18 maintains the same ultra-high inertness and wider pH range as the Shim-pack GIST C18, while providing rapid separations with symmetrical peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes. It is ideal for LC/ MS/MS analysis and enables MS-compatible buffers to be used due to extremely inert silica gel.
Bonded Phase
Octadecyl Groups 1.9 μm, 3 μm, 5 μm
Particle Size
Pore Size
20 nm
200 m 2 /g
Surface Area
Carbon Loading
9 %
End-capping
Yes
pH Range USP Code
1 to 10
L1
Analysis Examples
Peaks (100 μg/L each) Q1 > Q3
1. Acebutolol : 337.10 > 116.05 (+) 2. Atenolol : 267.25 > 145.00 (+) 3. Labetalol : 329.00 > 161.95 (+) 4. Nadolol : 310.05 > 254.00 (+) 5. Pindolol : 249.80 > 116.00 (+)
Conditions
Column
: Shim-pack GISS C18 (50 mmL × 2.1 mmI.D., 1.9 μm) (P/N: 227-30048-01)
Mobile Phase : A) 10 mM Ammonium Formate in Water B) 10 mM Ammonium Formate in Methanol
A/B = 70/30 - 0.3 min - 40/60 - 0.5 min - 0/100 - 0.1 min - 0/100 - 0.01 min - 70/30 - 0.5 min - 70/30, v/v
Flow Rate Col. Temp. Detection
: 0.6 mL/min
: 40 °C
: LC/MS/MS (ESI, Positive, Negative MRM)
Analytical Columns
I.D. (mm)
Particle Size (μm)
1.0
1.5
2.1
3.0
4.0
4.6
Length (mm)
30 50 75
227-30050-01 227-30050-02 227-30050-03 227-30050-04
227-30051-01 227-30051-02 227-30051-03 227-30051-04
227-30052-01 227-30052-02 227-30052-03 227-30052-04 227-30052-05 227-30052-06 227-30052-07 227-30058-01 227-30058-02 227-30058-03 227-30058-04 227-30058-05 227-30058-06 227-30058-07
227-30053-01 227-30053-02 227-30053-03 227-30053-04 227-30053-05 227-30053-06 227-30053-07 227-30059-01 227-30059-02 227-30059-03 227-30059-04 227-30059-05 227-30059-06 227-30059-07
227-30054-01 227-30054-02 227-30054-03 227-30054-04 227-30054-05 227-30054-06 227-30054-07 227-30060-01 227-30060-02 227-30060-03 227-30060-04 227-30060-05 227-30060-06 227-30060-07
227-30055-01 227-30055-02 227-30055-03 227-30055-04 227-30055-05 227-30055-06 227-30055-07 227-30061-01 227-30061-02 227-30061-03 227-30061-04 227-30061-05 227-30061-06 227-30061-07
3
100 125 150 250
-
-
227-30050-05 227-30050-06 227-30056-01 227-30056-02 227-30056-03 227-30056-04
227-30051-05 227-30051-06 227-30057-01 227-30057-02 227-30057-03 227-30057-04
30 50 75
5
100 125 150 250
-
-
227-30056-05 227-30056-06
227-30057-05 227-30057-06
Pg A-42
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