UHPLC/HPLC Columns
Shim-pack GIS C18
High Retentivity, Lower Column Back Pressure Widely used octadecyl bonded silica gel enables the Shim- pack GIS C18 to have strong hydrophobic interaction and low absorption of ionic compounds. In addition, highly uniform particles ensure stable mobile phase delivery and outstanding low pressure.
Bonded Phase
Octadecyl Groups
Particle Size
2µm, 3µm, 4µm, 5µm, 10µm
Pore Size
10 nm
450 m 2 /g
Surface Area
Carbon Loading
15%
End-capping
Yes
Shim-pack GIS C18 is ideal for preparative separations. Higher surface area silica and strong retentivity provide high preparative loading capacity without sacrificing peak shape.
pH Range USP Code
2 to 7.5
L1
Analysis Examples
Peaks 1. Tartrazine 2. Amaranth
7.6 mg/L 3.8 mg/L 7.6 mg/L 3.8 mg/L
10. Ponceau SX
5.3 mg/L 5.3 mg/L 3.0 mg/L 3.0 mg/L 7.6 mg/L 5.3 mg/L 3.0 mg/L 7.6 mg/L 3.0 mg/L
11. Orange I
3. Ingigocarmine 4. New coccine
12. Fast green FCF 13. Brilliant blue FCF 14. Ponceau 3R 15. Erythrosine 16. Azure Blue VX
5. Sunset Yellow FCF 5.3 mg/L 6. Naphthol Yellow S 7.6 mg/L
7. Uranine
3.8 mg/L 5.3 mg/L 7.6 mg/L
8. Allura Red AC
17. Orange II 18. Acid red
9. Ponceau R
Conditions
Column : Shim-pack GIS C18 (150 mmL. × 4.6 mmI.D, 4 μm) (P/N: 227-30100-07) Mobile Phase : A) 10 mmol/L Disodium phosphate buffer solution (pH 6.9) B) Acetonitrile A/B = 90/10 - 50 min - 65/35 Flow Rate : 1.0 mL/min Col. Temp. : 40 ºC Detection : UV 270 nm Injection Vol. : 10 μL
Analytical Columns
I.D. (mm)
Particle Size (μm)
1.0
1.5
2.1
3.0
4.0
4.6
Length (mm)
33 50 75
227-30095-01 227-30095-02 227-30095-03 227-30095-04
227-30096-01 227-30096-02 227-30096-03 227-30096-04
227-30096-05 227-30096-06 227-30096-07 227-30096-08 227-30096-09 227-30096-10 227-30096-11 227-30097-01 227-30097-02 227-30097-03 227-30097-04 227-30097-05 227-30097-06 227-30097-07 227-30097-08 227-30103-01 227-30103-02 227-30103-03 227-30103-04 227-30103-05 227-30103-06 227-30103-07 227-30103-08
227-30096-12 227-30096-13 227-30096-14 227-30096-15 227-30096-16 227-30096-17 227-30096-18 227-30098-01 227-30098-02 227-30098-03 227-30098-04 227-30098-05 227-30098-06 227-30098-07 227-30098-08 227-30104-01 227-30104-02 227-30104-03 227-30104-04 227-30104-05 227-30104-06 227-30104-07 227-30104-08
227-30096-19 227-30096-20 227-30096-21 227-30096-22 227-30096-23 227-30096-24 227-30096-25 227-30099-01 227-30099-02 227-30099-03 227-30099-04 227-30099-05 227-30099-06 227-30099-07 227-30099-08 227-30105-01 227-30105-02 227-30105-03 227-30105-04 227-30105-05 227-30105-06 227-30105-07 227-30105-08
227-30096-26 227-30096-27 227-30096-28 227-30096-29 227-30096-30 227-30096-31 227-30096-32 227-30100-01 227-30100-02 227-30100-03 227-30100-04 227-30100-05 227-30100-06 227-30100-07 227-30100-08 227-30106-01 227-30106-02 227-30106-03 227-30106-04 227-30106-05 227-30106-06 227-30106-07 227-30106-08
3
100 125 150 250
- - - - - - - - - - - -
- - - - - - - - - - - -
30 33 50 75
4
100 125 150 250
30 33 50 75
227-30101-01 227-30101-02 227-30101-03 227-30101-04
227-30102-01 227-30102-02 227-30102-03 227-30102-04
5
100 125 150 250
-
-
227-30101-05 227-30101-06
227-30102-05 227-30102-06
Pg A-44
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