UHPLC/HPLC Columns
Shim-pack GIS C18-P
High Steric Selectivity Shim-pack GIS C18-P is designed with a polymerically bonded octadecy group, which provides high steric selectivity for separation of planar and non-planar compounds. It achieves complete baseline separation of structurally similar compounds such as vitamin D2 and D3 because of the planarity recognition capability. Shim-pack GIS C18-P is also ideal for the HPLC analysis of 16 PAH compounds listed as target pollutants by the U.S. EPA.
Bonded Phase
Octadecyl Groups
Particle Size
3 µm, 5 µm
Pore Size
10 nm
450 m 2 /g
Surface Area
Carbon Loading
29 %
End-capping
-
pH Range USP Code
2 - 7.5
L1
Analysis Examples
Due to increased retention of planar structural compounds, Shim-pack GIS C18-P shows different selectivity compared to Shim-pack GIS C18.
Peaks 1. Phenanthro[3,4-c]phenanthrene (PhPh)
2. Tetrabenzonaphthalene (TBN)
3. Benzo[a]pyrene (BaP)
Conditions
Column
: 250 mmL. × 4.6 mmI.D., 5 µm
Mobile Phase : A) Water
B) Acetonitrile A/B = 15/85 (v/v)
Flow Rate Col. Temp. Detection Samples
: 2.0 mL/min
: 30 ºC
: UV 254 nm
: Standard Reference Material 869
Peaks (100 μg/L each) 1. Vitamin D2 (Calciferol) 2. Vitamin D3 (Cholecarciferol)
Conditions
Column
: Shim-pack GIS C18-P (250 mmL. × 4.6 mmI.D., 5 µm) (P/N: 227-30557-07)
Mobile Phase : Acetonitrile Flow Rate : 1.5 mL/min Col. Temp. : 40 ºC Detection : UV 265 nm Injection Vol. : 200 µL
Pg A-46
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