UHPLC/HPLC Columns
Shim-pack GIST Phenyl
Extremely Strong π-π Interactions The extremely unique phase characteristics of Shim-pack GIST Phenyl are critical to resolving compounds that could not be separated with a C18 or C8 phase column. In addition to π-π interactions, Shim-pack GIS Phenyl provides hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. As the phenyl groups are directly bonded to the silica gel, Shim- pack GIS Phenyl is also capable of the analysis of structural isomers due to its high stereo-selectivity.
Bonded Phase
Phenyl Group
Particle Size
2 μm, 3 μm, 5 μm
Pore Size
10 nm
350 m 2 /g
Surface Area
Carbon Loading
10 %
End-capping
-
pH Range USP Code
2 to 7.5
L11
Analysis Examples
Peaks (0.1 mg/L each)
1. Hydrocortisone 2. Prednisolone
Conditions
Column
: Shim-pack GIST Phenyl (50 mmL. × 2.1 mmI.D., 2 μm) (P/N: 227-30207-02)
Mobile Phase : A) 0.05 % Formic acid in Water B) 0.05 % Formic acid in Methanol A/B = 60/40, v/v Flow Rate : 0.6 mL/min Col. Temp. : 40 ºC Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : 5 μL
Analytical Columns
I.D. (mm)
Particle Size (μm)
1.0
1.5
2.1
3.0
4.0
4.6
Length (mm)
20 30 50 75
-
-
227-30211-01 227-30211-02 227-30211-03 227-30211-04 227-30211-05 227-30211-06 227-30211-07 227-30217-01 227-30217-02 227-30217-03 227-30217-04 227-30217-05 227-30217-06 227-30217-07
227-30212-01 227-30212-02 227-30212-03 227-30212-04 227-30212-05 227-30212-06 227-30212-07 227-30218-01 227-30218-02 227-30218-03 227-30218-04 227-30218-05 227-30218-06 227-30218-07
227-30213-01 227-30213-02 227-30213-03 227-30213-04 227-30213-05 227-30213-06 227-30213-07 227-30219-01 227-30219-02 227-30219-03 227-30219-04 227-30219-05 227-30219-06 227-30219-07
227-30214-01 227-30214-02 227-30214-03 227-30214-04 227-30214-05 227-30214-06 227-30214-07 227-30220-01 227-30220-02 227-30220-03 227-30220-04 227-30220-05 227-30220-06 227-30220-08
227-30209-01 227-30209-02 227-30209-03 227-30209-04 227-30209-05 227-30209-06
227-30210-01 227-30210-02 227-30210-03 227-30210-04 227-30210-05 227-30210-06
3
100 150 250
20 30 50 75
-
-
227-30215-01 227-30215-02 227-30215-03 227-30215-04 227-30215-05 227-30215-06
227-30216-01 227-30216-02 227-30216-03 227-30216-04 227-30216-05 227-30216-06
5
100 150 250
Pg A-52
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