Consumables Digital Catalogue

UHPLC/HPLC Columns

Shim-pack GIST PFPP

Strong retention for highly polar basic compounds

Different from other Shim-pack Series PFPP (pentafluorophenylpropyl) columns, this PFPP column is end-capped so it provides a different selectivity. Durability is a challenge for PFPP columns. However, Shim-pack GIST PFPP provides both high durability, which is a characteristic of the Shim-pack GIST Series, and separation characteristics of PFPP via a unique, advanced end-capping technology. Generally, PFPP columns retain more highly polar basic compounds. This is more prominent in Shim-pack GIST PFPP and it also provides an extremely high retention performance when compared to other commercially- available PFPP columns.

Bonded Phase

Pentafluorophenyl propyl Group

Particle Size

3μm, 5μm

Pore Size

10 nm

350 m 2 /g

Surface Area

Carbon Loading

10 %

End-capping

No

pH Range USP Code

2 to 7.5

L43

Analysis Examples

Peaks (100 µg/L each)

Analysis of Antihistamines

1. Ketotifen 2. Epinastine 3. Chlorpheniramine 4. Cetirizine

5. Triprolidine 6. Hydroxyzine 7. Fexofenadine 8. Azelastine

9. Loratidine 10.Homoclorcyclizine 11.Clemastine

Conditions

Column

: Shim-pack GIST-HP PFPP (3 μm, 150 x 2.1 mm I.D.) (P/N: 227-30890-05)

Mobile Phase : A) Acetonitrile

B) 0.1% Formic acid in water A/B = 40/60, v/v

Flow Rate Col. Temp. Detection

: 0.2 mL/min

: 40 ºC

: LCMS-8030 Plus (ESI, Positive, SRM)

Injection Vol. : 1 μL Sample

: Standard

Analytical Columns

I.D. (mm)

Particle Size (μm)

1.0

1.5

2.1

3.0

4.0

4.6

Length (mm)

20 30 50 75

-

-

227-30858-01 227-30858-02 227-30858-03 227-30858-04 227-30858-05 227-30858-06 227-30858-07 227-30858-08 227-30864-01 227-30864-02 227-30864-03 227-30864-04 227-30864-05 227-30864-06 227-30864-07 227-30864-08

227-30859-01 227-30859-02 227-30859-03 227-30859-04 227-30859-05 227-30859-06 227-30859-07 227-30859-08 227-30865-01 227-30865-02 227-30865-03 227-30865-04 227-30865-05 227-30865-06 227-30865-07 227-30865-08

227-30860-01 227-30860-02 227-30860-03 227-30860-04 227-30860-05 227-30860-06 227-30860-07 227-30860-08 227-30866-01 227-30866-02 227-30866-03 227-30866-04 227-30866-05 227-30866-06 227-30866-07 227-30866-08

227-30861-01 227-30861-02 227-30861-03 227-30861-04 227-30861-05 227-30861-06 227-30861-07 227-30861-08 227-30867-01 227-30867-02 227-30867-03 227-30867-04 227-30867-05 227-30867-06 227-30867-07 227-30867-08

227-30856-01 227-30856-02 227-30856-03 227-30856-04

227-30857-01 227-30857-02 227-30857-03 227-30857-04

3

100 125 150 250

-

-

227-30856-05 227-30856-06

227-30857-05 227-30857-06

20 30 50 75

-

-

227-30862-01 227-30862-02 227-30862-03 227-30862-04

227-30863-01 227-30863-02 227-30863-03 227-30863-04

5

100 125 150 250

-

-

227-30862-05 227-30862-06

227-30863-05 227-30863-06

Pg A-56

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