UHPLC/HPLC Columns
Shim-pack GIS HILIC
Ideal for the Separation of Highly Polar Basic Compounds Shim-pack GIS HILIC is designed for Hydrophilic Interaction Liquid Chromatography (HILIC). It is chemically bonded with a diol group, which provides excellent peak shape for basic and neutral polar compounds. Bonded Phase Particle Size Pore Size
Diol Groups 3 µm, 5 µm
10 nm
450 m 2 /g
Surface Area
Carbon Loading
20 %
In addition, HILIC is a variation of normal phase mode. It is capable of using organic solvents mixed with water as mobile phase, while normal phase mode uses non- aqueous organic solvents. In HILIC, the higher the organic concentration in the solvents, the greater is the retention of highly polar compounds.
End-capping
-
pH Range USP Code
2 to 7.5
L20
Analysis Examples
Peaks 1. Allantoin
Conditions
Column
: Shim-pack GIS HILIC (250 mmL. × 3.0 mmI.D., 5 µm) (P/N: 227-30639-07)
Mobile Phase : A) 10 mmol/L Ammonium acetate B) Acetonitrile A/B = 5/95 (v/v) Flow Rate : 0.4 mL/min Col. Temp. : 40 ºC Detection : UV 210 nm
Peaks (500 mg/L each) 1. Taurine 2. Inositol
Conditions
Column
: Shim-pack GIS HILIC (150 mmL. × 3.0 mmI.D., 5 µm) (P/N: 227-30639-06)
Mobile Phase : A) Water
B) Acetonitrile A/B = 20/80 (v/v)
Flow Rate Col. Temp. Detection
: 0.4 mL/min
: 40 ºC
: RID (35 ºC, positive)
Injection Vol. : 20 μL
Pg A-58
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