UHPLC/HPLC Columns
Shim-pack GIST NH2
Ideal for Sugar Analysis New high-purity porous spherical silica chemically bonded with the aminopropyl group ensures the superior stability of Shim-pack GIST NH2. It is capable of the analysis of vitamin E or simultaneous analysis of sugars that are hard to separate in reversed phase mode. In addition, due to being primarily amine-bound, Shim-pack GIST NH2 can analyze sugars with no separation of anomers, even under low-temperature conditions. Furthermore, Shim-pack GIST NH2 delivers highly reliable reproducibility and stability with accurate qualitative and quantitative results.
Bonded Phase
Aminopropyl Group
Particle Size
3 μm, 5 μm
Pore Size
10 nm
Surface Area
350 m 2 /g
Carbon Loading
7 %
End-capping
-
pH Range USP Code
2 to 7.5
L8
Analysis Examples
Peaks 1. Fructose 2. Glucose 3. Sucrose 4. Maltose
Conditions
Column
: Shim-pack GIST NH2 (250 mmL. × 4.6 mmI.D., 5 μm) (P/N: 227-30302-08)
Mobile Phase : A) Water
B) Acetonitrile A/B = 25/75, v/v
Flow Rate Col. Temp.
: 1.0 mL/min
: 40 ºC
Detection : RID Injection Vol. : 5 μL
Peaks (25 mg/L each)
1. α -Tocopherol 2. β -Tocopherol 3. γ -Tocopherol 4. δ -Tocopherol
Conditions
Column
: Shim-pack GIST NH2 (250 mmL. × 4.6 mmI.D., 5 μm) (P/N: 227-30302-08)
Mobile Phase : A) n -Hexane
B) Ethyl acetate A/B = 70/30, v/v
Flow Rate Col. Temp. Detection
: 1.0 mL/min
: 40 ºC
: UV 290 nm
Injection Vol. : 10 μL
Pg A-60
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