UHPLC/HPLC Columns
Shim-pack GIS CN
Suitable in Either Reversed Phase or Normal Phase Mode Shim-pack GIS CN is capable of either normal phase or reversed phase analysis. Cyanopropryl groups bonded to silica gel with high density increases the difference recognition of hydrophilicity and the stability. Due to no end-capping, it is capable of analysis utilizing cyano group characteristics. Bonded Phase Particle Size Pore Size Surface Area Carbon Loading
Cyanopropryl Group
3 μm, 5 μm
10 nm
450 m 2 /g
14 %
End-capping
-
pH Range USP Code
2 to 7.5
In reversed phase mode, separation can be achieved for those compounds that could not be separated on straight- chain-alkyl columns, such as C18 or C8 bonded phases. When using the column for reversed phase mode, fully equilibrate the column before use.
L10
Analysis Examples
γ
Peaks 1. gamma-oryzanol 2. vanillin
Conditions
Column
: Shim-pack GIS CN (150 mmL. × 4.6 mmI.D, 5 μm) (P/N: 227-30263-06)
Mobile Phase : A) Hexane
B) 2-Propanol C) Acetic acid A/B/C = 94/5/1 (v/v/v)
Flow Rate Col. Temp. Detection
: 1.0 mL/min
: 30 ºC
: UV 320 nm
Injection Vol. : 1 μL
Peaks 1. Asparagine • H 2 O (0.75 mg/mL) 2. Aspartic acid (0.75 mg/mL) 3. Fumaric acid (0.01 mg/mL) 4. Maleic acid (0.01 mg/mL)
Conditions
Column : Shim-pack GIS CN (250 mmL. × 4.6 mmI.D., 5 μm) (P/N: 227-30263-07) Mobile Phase : 20 mmol/L Monopotassium phosphate buffer solution (pH 4.0) Flow Rate : 1.0 mL/min Col. Temp. : 40 ºC Detection : UV 210 nm Injection Vol. : 5 μL
Pg A-64
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