Shim-pack FC-ODS
Shortens the Analysis Time Using a Conventional System Shim-pack FC-ODS is an ideal column to shorten your analysis time using conventional HPLC. Its innovative surface structure and optimized packing method also enable outstanding resolution. Particle size is 3 μm, but the performance of a Shim-pack FC-ODS is equivalent to a 2 μm column while the resolution is twice as that of a 5 μm column. Therefore, Shim-pack FC-ODS can not only shorten analysis times, but also provide a higher number of theoretical plates. Particle Size Pore Size Surface Area Carbon Loading Pressure Tolerance Pore Volume End-capping Bonding Type
3 μm
12 nm
315 m 2 /g
18%
20 MPa
1 mL/g
Yes
Monomeric
pH Range USP Code
1.5 - 9
L1
Analysis Examples Shim-pack FC-ODS separates components by hydrophobic interaction like other ODS columns. It is possible to change to Shim-pack FC-ODS from other ODS columns under the same analytical conditions. On the other hand, hydrophilic interaction (hydrogen bond, coordination bond) has been restricted to a minimum, which ensures significant efficiency when analyzing basic compounds. In addition, Shim-pack FC-ODS has higher steric selectivity (capability to recognize the difference of steric structures), making it possible
to separate some components that are difficult to retain in other ODS columns. Shim-pack FC-ODS is available in three lengths to suit analysis objectives.
30mm Recommended for use in high-throughput analysis of samples that do not have a complex matrix. 75mm
Recommended for shortening the analysis time to that of a 150 mm column. Because Shim-pack FC-ODS retains a similar number of theoretical plates as a 150 mm column, it is possible to obtain the same result within about half of the time without changing the conditions. (In the case of gradient analysis, it is necessary to change the concentration.) 150mm Recommended for analyzing samples that are difficult to be retained in other 150 mm ODS columns.
High-Throughput Analysis
Conditions
Column
: Shim-pack FC-ODS (30 mmL. × 4.6 mmI.D., 3 μm) (P/N: 228-40511-91)
Mobile Phase : Water/Acetonitrile = 55/45 (v/v) Flow Rate
: 3.0 mL/min (Column Pressure ca.8MPa)
Col. Temp. Detection Instrument
: 50 ºC
: 254 nm Response 1, AuxRNG×2
: LC-2010+C-R8A
10 times repeated analysis in 6.5 minutes is possible.
Pg A-67
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