Bio LC Columns
Shim-pack Bio LC Columns Solution for Analysis of Peptides, Oligonucleotides and Biopharmaceuticals The accurate analysis of biopharmaceuticals compounds is required for developing higher quality biopharmaceuticals. Shim-pack Bio Diol and IEX columns will improve the accuracy of the characterization of peptides, oligonucleotide and biopharmaceuticals.
Shim-pack Bio Diol : Size Exclusion Chromatography Columns
With different pore sizes, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb, oligonucleotides and carbohydrates.
Rapid mAb Aggregate Analysis using 2μm Shim-pack Diol-300 column
Shim-pack Bio Diol
Diol-60
Diol-120
Diol-200
Diol-300
Particle Ligand
Silica
Dihydroxypropyl(Diol)
Particle Size
3 μm, 5 μm
2 μm, 3 μm, 5 μm
Pore Size pH Range
6 nm 12 nm 20 nm 30 nm
5.0 - 7.5
below 10,000
1,000 - 100,000
5,000 - 300,000
20,000 - 1,000,000
Molecular
By reducing the particle size from 5 μm to 2 μm, the resolution between aggregates and monomers was greatly improved. Furthermore, by reducing the column length from 300 mm to 150 mm using a 2 μm particle, 50% less run time was achieved, while maintaining resolution as compared to the original method with a 5 μm, 4.6×300 mm column.
Conditions
Column
: Shim-pack Bio Diol-300
Eluent
: 0.1 M KH2PO4-K2HPO4 (pH 7.0) with 0.2 M NaCl
Flow rate : 0.2 mL/min Column Temp. : Ambient Detection : UV 280 nm Sample
: Humanized monoclonal IgG1
Shim-pack Bio IEX : Ion Exchange Chromatography Columns Shim-pack Bio IEX Columns are available in Q (quaternary ammonium) and SP (sulfopropyl) chemistries and are based on porous (Q and SP columns) and non-porous (Q-NP and SP-NP columns) hydrophilic polymers with low nonspecific adsorption. The porous particles offer excellent binding capacity with exceptionally high efficiency and the non-porous particles offer high efficiency and exceptional resolution.
Rapid mAb Aggregate Analysis using 2μm Shim-pack Diol-300 column
Shim-pack Bio IEX
Q-NP
SP-NP
Q
SP
hydrophilic non-porous polymer
hydrophilic porous polymer
Particle
Particle Size
3 μm, 5 μm
5 μm
Ligand
- CH 2 N
+ (CH
3 ) 3
- (CH 2 ) 3 SO 3 - - CH 2 N
+ (CH
3 ) 3
- (CH 2 ) 3 SO 3 -
pH Range
2 - 12
Conditions
Column
: Shim-pack Bio IEX Q-NP 5 μm, 4.6×100 mm (P/N : 227-31003-03)
Mobile Phase A : 10 mM NaOH Mobile Phase B : 10 mM NaOH with 1.0 M NaClO4 Gradient
: 25 55%B (0-15 min), 100%B (15-20 min)
Flow Rate
: 1.0 mL/min
Column Temp.
: 25 °C
Detection Inj. Volume
: UV 260 nm
: 4 μL (5 nmol/mL)
Pg A-76
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