Bio LC Columns
Shim-pack Bio HIC: Hydrophobic Interaction Chromatography Column
Antibody-drug conjugate (ADC) is a next-generation antibody drug that is expected to provide strong effects in a wide range of therapeutic treatments as compared to existing chemotherapeutic agents. The drug-to-antibody ratio (DAR) indicates the ratio of addition of the drug that directly influences both efficacy and potential toxicity of ADCs. Analysis of DAR is extremely important when judging important quality attributes of ADCs as it may significantly affect properties such as stability and agglutination. Hydrophobic interaction chromatography (HIC) is suitable for separation of analytes with slightly different hydrophobic properties such as antibody-drug conjugates (ADCs) with a different drug-to-antibody ratio (DAR). Using the Shim-pack Bio HIC, the DAR of ADCs can be analysed with a high separation capacity.
Drug to Antibody Ratio (DAR) analysis of ADCs
mV
DAR4
30
Conditions
Column : Shim-pack Bio HIC, 4 μm, 4.6×100mm Mobile Phase : A) 50 mM NaH 2 PO 4 -Na 2 HPO 4 (pH 7.0) containing 1.5 M (NH 4 ) 2 SO 4 /2-propanol (95/5)
20
B) 50 mM NaH 2 PO 4 -Na 2 HPO 4 (pH 7.0)/2-propanol (80/20) 0%B (0-1 min), 0-100%B (1-15 min), 100%B (15-20 min)
Flow Rate Col. Temp. Detection
: 1.0 mL/min
: 25 ºC
DAR6
DAR2
: UV at 280 nm
10
Injection Vol. : 5 µL Sample
DAR8
: Cystein-conjugated ADC mimic (5 mg/mL)
DAR0
0
0
5
10
15
20 min
Shim-pack Bio HIC Butyl
P/N
Dimensions
227-31174-01
4 μm, 100 x 4.6 mm I.D
Pg A-78
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