Various molecules give an increase in the peak intensity on Nexera Mikros TM LC-MS/MS platform with an MC PLONAS column.
Shim-pack MC PLONAS C18
3000
6.0e2
2500
5.0e2
Micro Semi-micro
2000
4.0e2
1500
3.0e2
Aldosterone 1 pg/mL
1000
2.0e2
500
1.0e2
0
0.0e0
5(
min)
12
34
1.02
.0
3.04
.0
5.0
Column
: Shim-pack MC PLONAS C18 (0.2 mm I.D. x 100 mm L., 2.7 μm) *Semi-micro data acquired with the 2.1 mm I.D. column which has the same chemical profile as an MC PLONAS column.
Mobile phase
: A: Water, B: Methanol, gradient elution
Flow rate:
: 4 μm/min
Temperature
: 50 °C
Injection volume
: 1 μL
MS detection
: LCMS-8060, with Micro-ESI source, ESI (-)
Shim-pack MC PLONAS C8
1. All- trans Retinol (A) 2. Epoxy-Menaquinone-4 (epoxy-MK-4) 3. Menaquinone-4 (MK-4) 4. -Tocopherol (E) 5. Epoxy-Phylloquinone (epoxy-K1) 6. Phylloquinone (K1) 7. Menaquinone-7 (MK-7) 1
6
5
4
7
3
2
Column
: Shim-pack MC PLONAS C8 (0.2 mm I.D. x 100 mm L., 2.7 μm)
Mobile phase : A: Ammonium acetate aq.
B: Ammonium acetate in methanol, gradient elution
Flow rate
: 4 μL/min
Temperature : 50 °C Injection volume : 1 μL MS detection : LCMS-8060, with Micro-ESI source, ESI (-)
0.0 1.02 .0 3.04 .0 5.06 .0 7.08 .0 9.0 10.0 11.0 12.0 13.0m in
20 pg on column * each peak is in full scale intensity
Pg A-81
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