Shim-pack Diol Series Shim-pack Diol series is a kind of gel filtration chromatography (GFC) column. GFC is used to separate water-soluble high polymers such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and aqueous mobile phase. Shim-pack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. Due to the hydrophilic hydroxyl group, Shim-pack Diol series can be used in high-speed GFC and provide sharp peaks during the analysis of protein and biochemicals (such as enzymes).
Product Information
Particle Size (μm)
Dimensions (Length × I.D., mm)
Column
Stationary Phase
P/N
250 × 7.9 500 × 7.9 250 × 7.9 500 × 7.9
228-14775-91 228-14775-92 228-14776-91 228-14776-92 228-16367-91
Shim-pack Diol-150
Diol group
5
Shim-pack Diol-300
Diol group
5
Pre-column Diol *
Diol group
10
50 × 4.0
* Installed between the liquid pump and the sample injector to protect the Shim-pack Diol column.
Analysis Examples
Calibration Curves
Peaks 1. Blue dextran 2000 2. Thyroglobulin 3. y-Globulin 4. Transferrin 5. Human serum albumin 6. Ovalbumin
7. β -Lactoglobulin 8. Chymotrypsin
9. Myoglobin 10. Lysozyme
11. Ribonuclease A 12. Cytochrome C 13. Gly-Tyr
Conditions
Column
: Shim-pack Diol Series (500 mmL × 7.9 mmI.D., 5 μm)
Mobile Phase : A) 10mM phosphate buffer solution (pH 7) B) 0.2M sodium sulfate Flow Rate : 1.0 mL/min Col. Temp. : Ambient Detection : UV 280 nm
Analysis of Protein Standard
Conditions
Peaks 1. Glutamate dehydrogenase 2. Lactate dehydrogenase 3. Enolase 4. Adenylate kinase 5. Cytochrome C
Column
: Shim-pack Diol-300 (500 mmL × 7.9 mmI.D., 5 μm) (P/N: 228-14776-92)
Mobile Phase : A) 10mM phosphate buffer solution (pH 7) B) 0.1M sodium chloride Flow Rate : 1.0 mL/min Col. Temp. : Ambient Detection : UV 280 nm
Pg A-85
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