Mastro TM SP Ion-paring reagents are required to analyze the highly-polar compound such as phosphate groups. However, ion-paring reagents are not ideal for LCMS applications as they as difficult to remove from the flow lines. Mastro TM SP columns are ideal for the analysis of such compounds without the use of ion-paring reagents.
Analytical condition
Instruments
: Shimadzu Nexera/LCMS-8050
Column : Mastro™ SP (2.1 mmI.D × 100 mmL., 5 μm) Mobile phase : A) 10 mM Ammonium acetate/ACN=90/10 Mobile phase : B) 50 mM Ammonium acetate/ACN=80/20 0% B (0-4.0 min) 60% B (9.0 min)
00% B (11.0-20.0 min) 0% B (20.1-25.0 min)
T. Flow : 0.5 mL/min Nebulizing gas : 3.0 L/min Drying gas : 12 L/min DL Temp. : 250 ºC BH Temp. : 400 ºC Injection Vol. : 3 μL
Length 100 mm
Particle Size (μm)
I.D (mm)
5.0
2.1
SSOT-G10005
Mastro2™ PFP Mastro2 PFP is the column packed with PFP (Penta Fluoro Phenyl) that has high retention capability. Generally, PFP columns have efficiency for retaining and separating regioisomers and halogen compounds that are difficult to be analysed with C18 column.
Separation of cresol
Analytical conditions
Mastro2™ PFP Typical PFP
Instrument
: NexeraX2_SPD20A(Semi-micro cell)
α 2,3 α 3,4
1.06 1.10 1.50 2.50
1.10 1.82 0.59 0.92
Column
: Mastro2™ PFP (150 mmL. × 2.0 mm I.D. 3 μm) Typical PFP* 1 (150 mmL. × 2.1 mm I.D. 3 μm)
Rs1 Rs2
Mobile phase : H 2 O / CH 3 OH = 60 / 40 Flow rate : 0.2 mL/min Detection : UV 254 nm Column oven : 40 ºC Sample
: 1. Uracil, 2. p-Cresol, 3. m-Cresol, 4. o-Cresol
* 1 Metal free column
Mycotoxin components (3-ADON, 15-ADON) analysis
Analytical conditions
Instrument
: NexeraX2_SPD20A (Semi-micro cell)
Column
: Mastro2™ PFP (150 mm L, × 2.0 mm I.D. 3 μm)
Mobile phase : A) 10 mM CH 3 COONH 4 in H 2 O B) 2% CH 3 COOH in CH 3 OH Gradient condition Flow rate : 0.4 mL/min Detection : UV 254 nm Column oven : 40 ºC Sample : 1. 15-ADON, 2. 3-ADON
Length
Particle Size (μm)
I.D (mm)
100 mm
150 mm
3.0
2.0
370-01015-64
370-01015-84
Pg A-89
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