Analysis Examples
Tocopherol Isomers Isomers and structurally similar compounds that are difficult to separate can be analyzed by Nexera UC and Shim-pack UC series at a high speed. In the example below, four kinds of tocopherol isomers were analyzed by Nexera UC and Shim-pack UC Sil. As shown below, analysis time was reduced to one-third the time analysis takes with a conventional HPLC method while resolution was improved.
Phospholipid Classes
The example above shows the result of the analysis of phospholipid classes using Shim-pack UC Diol with Nexera UC. The ODS-based Shim-pack UC GIS II can also be used to analyze molecular species of phospholipids with the same modifiers. Nexera UC and Shim-pack UC series are ideal for analyzing complex samples.
Comparison of retention and resolution of tocopherol isomers between LC conditions and SFC ones
Preparative Columns
I.D. (mm)
Particle Size (μm)
Column
10
20
28
Length (mm)
Shim-pack UC-HyP
5 5 5 5 5 5 5 5 5 5 5 5
250
227-32600-03 227-32600-06 227-32601-03 227-32601-06 227-32602-03 227-32602-06 227-32603-03 227-32603-06 227-32604-03 227-32604-06 227-32605-03 227-32605-06
227-32600-04
227-32600-05
Guard Column
20
-
-
Shim-pack UC-Py
250
227-32601-04
227-32601-05
Guard Column
20
-
-
Shim-pack UC-PBr
250
227-32602-04
227-32602-05
Guard Column
20
-
-
Shim-pack UC-Choles
250
227-32603-04
227-32603-05
Guard Column
20
-
-
Shim-pack UC-PyE
250
227-32604-04
227-32604-05
Guard Column
20
-
-
Shim-pack UC-Triazole
250
227-32605-04
227-32605-05
Guard Column
20
-
-
Shim-pack UC-Sil II
5
250
227-32607-03
227-32607-04
227-32607-05
Guard Column
5
20
227-32607-06
-
-
Shim-pack UC-Diol II
5
250
227-32606-03
227-32606-04
227-32606-05
Pg A-93
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