Wildlife Diversity Annual Report 2025

MOLLUSKS

MOLLUSKS Development of Genetic Tools to Aid in the Restoration of Two Federally Endangered Mussel Species, the Tar River Spinymussel and Yellow Lance

by Dr. Heather Evans, Conservation Genetics Manager

T he NCWRC’s Genetics Program recently completed development of an eDNA assay for Spinymussels. This assay will allow for monitoring and detection of Tar River Spinymussel and James River Spinymussel, both Federally Endangered species. The assay will allow us to utilize a systematic eDNA sampling regime to identify reaches that are likely to contain wild Tar River Spinymussels. The results of this work will potentially result in increased broodstock collections, identification of new sites for broodstock surveys, and optimization of survey efforts dedicated to these species. Additionally, staff completed development of two genetic panels that will be used for mon- itoring of stocked Tar River Spinymussels and Yellow Lance. The panels will allow for high throughput, accurate detection of hatchery-bred individuals. They can be used to monitor percent hatchery contribution across populations as propagation, augmentation and reintro- duction efforts continue. The panels can also be used to monitor genetic diversity and popula- tion structure with ongoing stocking efforts, allowing hatchery managers to protect important allelic diversity in their breeding program.

RACHAEL HOCH/NCWRC

Staff collecting a swab from the foot of a Tar River Spinymussel brood mussel for use in genetic panel development.

68 2025 WILDLIFE DIVERSITY PROGRAM ANNUAL REPORT

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