Site-Controlled Incorporation of ATRP Initiators into nucleic acids for the controlled synthesis of DNA- or RNA-polymer hybrids Jaepil Jeong 1,2 , Grzegorz Szczepaniak 1,3 , Subha R. Das 1,2* and Krzysztof Matyjaszewski 1* 1 Department of Chemistry, Carnegie Mellon University, USA, 2 Center for Nucleic Acids Science & Technology, Carnegie Mellon University, USA, 3 University of Warsaw, Faculty of Chemistry, Poland The combination of synthetic polymers with nucleic acids opens new possibilities for developing DNA-or RNA- based materials with novel functions. In this study, we have developed a serinolic ATRP initiator-modified phosphoramidite ( SBiB ) that can be incorporated during solid-phase synthesis of DNA and RNA with precise location- and number-control. 1 We have also created an acylation reagent that enables the direct incorporation of an ATRP initiator into short synthetic RNA and natural biomass RNA derived from torula yeast. 2 Following the synthesis of the DNA or RNA macroinitiators using SBiB or initiator-modified acylation reagent, polymerization enabled the fabrication of DNA or RNA functionalized with polymers at the desired positions. We envision that this approach enables the precise synthesis of complicated nucleic acid-polymer structures, significantly expanding the range of functions of polymer biohybrid materials. References 1. J. Jeong, G. Szczepaniak, S. R. Das, and K. Matyjaszewski, Expanding the Architectural Horizon of Nucleic Acid-Polymer Biohybrids by Site-Controlled Incorporation of ATRP Initiators in DNA and RNA. Chem , accepted J. Jeong, X. Hu, H. Murata, G. Szczepaniak, M. Rachwalak, A. Kietrys, S. R. Das, and K. Matyjaszewski, RNA-Polymer Hybrids via Direct and Site- Selective Acylation with the ATRP Initiator and Photoinduced Polymerization, J. Am. Chem. Soc. , 2023, 145 , 14435–14445.
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© The Author(s), 2023
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