Preparation and investigation of polysaccharide-based highly efficient antibacterial micro-composite for efficient hemostasis Lamiya Hassan Tithy and M. Tarik Arafat * Department of Biomedical Engineering, Bangladesh University of Engineering and Technology, Bangladesh * Designates corresponding author Excessive bleeding and bacterial inflammation are major worldwide problems[1][2][3]. The preparation of rapid hemostatic materials along with strong bactericidal properties for deep irregular hemorrhage still remains a challenge[4][5]. A novel micro-composite made with different natural polysaccharides chitosan and starch cross-linked with a polyphenolic was prepared via facile ionic gelation method. The chemical bonds between the constituent materials were verified by ATR-FTIR. The micro-composite material has excellent hemostatic properties and can concentrate blood cells, blood proteins, and clotting factors as well as can induce electrostatic interaction between positively charged chitosan and negatively charged blood cells. Blood clotting index (BCI), and blood cell adhesion were performed to verify its hemostatic capability in vitro. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were employed to find out the composite’s hemostatic mechanism. For the in vivo hemostatic study, mice hepatic and tail injury models were performed to evaluate the composite’s efficacy in real injury scenarios. These studies suggest that the prepared micro-composite can be employed in irregularly shaped wounds to halt bleeding. It was also observed that the composite showed almost similar clotting time in mice hepatic injury models compared with the commercial hemostatic granules CELOX. The composite also exhibited excellent bactericidal activity (>80%) in gram-positive Staphylococcus aureus, and gram-negative Escherichia coli in comparison with the commercial hemostatic granules CELOX TM , which does not have any antibacterial properties against gram-negative bacteria. Furthermore, excellent hemocompatibility, measured by hemolysis ratio (<3%), in vitro biodegradation of the prepared micro-composite was also confirmed. These results suggest that the micro-composite has great promise as an efficient hemostatic agent with substantial antibacterial properties to be used in emergency situations for preventing massive hemorrhage. References 1. D. R. Spahn, B. Bouillon, V. Cerny, J. Duranteau, D. Filipescu, and B. J. Hunt, “Guia europea sangrado y coagulación,” Crit. care , vol. 23, pp. 1–74, 2019. 2. D. Annane et al. , “Effects of fluid resuscitation with colloids vs crystalloids on mortality in critically ill patients presenting with hypovolemic shock - The CRISTAL randomized trial,” Jama , vol. 310, no. 17, pp. 1809–1817, 2013 3. D. S. Kauvar, R. Lefering, and C. E. Wade, “Impact of hemorrhage on trauma outcome: An overview of epidemiology, clinical presentations, and therapeutic considerations,” J. Trauma - Inj. Infect. Crit. Care , vol. 60, no. 6 SUPPL., pp. 3–11, 2006 4. Wang et al. , “Cellulose fibers-reinforced self-expanding porous composite with multiple hemostatic efficacy and shape adaptability for uncontrollable massive hemorrhage treatment,” Bioact. Mater. , vol. 6, no. 7, pp. 2089–2104, 2021 5. Y. Liang, B. Chen, M. Li, J. He, Z. Yin, and B. Guo, “Injectable Antimicrobial Conductive Hydrogels for Wound Disinfection and Infectious Wound Healing,
P96
© The Author(s), 2023
Made with FlippingBook Learn more on our blog