Fluorescent Probes: NBD & TopFluor TM
Product Nitrobenzoxadiazole (NBD) is a common fluorescent label, especially for lipids. NBD was discovered several decades ago but continues to be a popular choice for fluorescent labelling due to its desirable fluorescent characteristics. NBD shows maximum excitation and emission wavelengths of 465nm and 535nm, respectively. Nitrobenzoxadiazole (NBD)
NBD probes are environmentally sensitive and highly reactive to amines and thiols. This environmental sensitivity provides key advantages that facilitate biomolecular interactions and self- assembly within buffers and live systems. The strong electron withdrawing nature of the nitro groups results in NBD derivates able to undergo aromatic substitution (if a suitable leaving group is present) which has helped researchers develop a variety of different sensing motifs for biological nucleophiles. These key chemical properties result in a fluorophore that is easily chemically modified and can be attached to a variety of proteins as well as other biomolecules. Due to the ease at which NBD can be attached to biomolecules, it makes NBD compounds valuable assets in lipid membrane studies, lysosomal lipid body analysis, and for drug screening.
Product TopFluor TM
TopFluor (also referred to as BODIPY) is an extremely versatile fluorophore that can be structurally modified to fulfil a variety of roles. TopFluor is an extremely versatile fluorophore due to its chemical makeup and its high tolerance for substitutions which results in a compound that can be derivatized to suit a researcher’s needs. TopFluor-labeled lipids are great aids in studying the distribution of lipids, lipid diffusion rates, and lipid dynamics. TopFluor has a tunable fluorescence emission range from 500 - 700nm, high fluorescent quantum yields regardless of the solution, and a high level of photostability. TopFluor has also been used to study accessibility in the lipid bilayer, cellular localization, and membrane dynamics via fluorescence recovery. Studies have indicated that this fluorescent label alters the biochemical and biophysical properties of lipids less than some other fluorescent labels, such as NBD, while also maintaining a low toxicity, low polarity, and a high degree of biocompatibility.
Top Fluor TM
Top Fluor TMR TM
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