TESTING
laboratory to extract the viral RNA. This step involves breaking open the virus particles and isolating the RNA. 3. Reverse transcription: The extracted viral RNA is converted into complementary DNA (cDNA) using an enzyme called reverse transcriptase. This step allows for easier amplification of the viral genetic material. 4. PCR amplification: The cDNA is mixed with specific primers, DNA polymerase, and nucleotides in a process called PCR amplification. The primers are designed to target specific regions of the SARS- CoV-2 genome. The DNA polymerase enzyme amplifies the targeted viral genetic material through a series of heating and cooling cycles, creating millions of copies of the viral DNA. 5. Detection: The PCR amplification is monitored in real-time using fluorescent probes or dyes. If the virus is present in the patient's sample, the fluorescence signal will increase during the amplification cycles, indicating a positive result. If the virus is not present, there will be no significant increase in fluorescence, indicating a negative result. 4. 3. 5.
The result of a COVID PCR test is typically reported as positive or negative for SARS-CoV-2 infection. The test can be highly sensitive, detecting even small amounts of viral genetic material. However, it's important to note that a negative PCR test does not completely rule out COVID-19, especially if the test is performed during the early stages of infection or if the sample is not collected properly. COVID PCR tests are widely used for diagnostic purposes, contact tracing, surveillance, and monitoring the spread of the virus. They are typically performed in laboratories or clinical settings with specialized equipment and trained personnel. Rapid PCR tests with shorter turnaround times are also available in some settings, allowing for quicker results. CONTACT US TODAY: 236 W. Edison Rd Mishawaka, IN 46545 (800) 450-0040
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