CAMP EDVOTEK: 2024 SUMMER ACTIVITY BOOKLET

Welcome to Camp Edvotek, our Summer Science Camp that you can run in the comfort of your own home! We are thrilled to have you join us for a fun-filled journey of discovery and learning. Our camp counselors in the lab have curated resources for curious minds of all ages, where you’ll get to explore exciting scientific concepts through hands-on experiments, engaging activities, and YouTube videos. Whether your camper is a budding scientist or just loves to learn new things, we have something for everyone. Get ready to ignite imagination, satisfy curiosity, and have a blast as we dive into the fascinating world of biotechnology together. Be sure to follow along with us all summer at blog.edvotek.com. We can’t wait to embark on this adventure with you!

EDVOTEK® SUMMER CAMP

ACTIVITY BOOKLET

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EDVOTEK ® SUMMER CAMP ACTIVITY BOOKLET

WELCOME!

Welcome to CAMP EDVOTEK , our Summer Science Camp that you can run in the comfort of your own home! We are thrilled to have you join us for a fun-filled journey of discovery and learning. Our camp counselors in the lab have curated re- sources for curious minds of all ages, where you’ll get to explore exciting scientific concepts through hands-on experiments, engaging activities, and YouTube videos. Whether your camper is a budding scientist or just loves to learn new things, we have something for everyone. Get ready to ignite imagination, satisfy curiosity, and have a blast as we dive into the fascinating world of biotechnology together. Be sure to follow along with us all summer at blog.edvotek.com . We can’t wait to embark on this adventure with you!

FOLLOW EDVOTEK ® AND ENGAGE WITH US

VISIT OUR WEBSITE

View all our products, place orders, and more! www.edvotek.com

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FORENSIC

SCIENCE

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FORENSIC SCIENCE PRODUCTS AND RESOURCES:

RELATED EXPERIMENTS

RELATED RESOURCES EDVOTEK INFORMATIONAL VIDEO: DNA Profiling

Cat. #S-91 Whose Fingerprints Were Left Behind?

Students will learn to detect and analyze fingerprints and then use these techniques to solve a classroom crime.

Explore the use of fingerprint evi - dence in forensic science

• Create personal ink prints using our Fingerprint Report Cards™, identify key patterns, and practice dusting for prints • Compare classroom fingerprints to detected crime scene evidence

EDVOTEK LIVE VIDEO: Do You Have What It Takes to Solve a Crime? Using Forensic Blood Typing in the Teaching Laboratory

Cat. #191 Forensics Blood Typing Introduce students to some of the techniques used by forensics scientists for analyzing blood. • Understand the chemistry and the biology behind blood analysis techniques • Explore presumptive and confirmatory forensic tests • Perform a Kastle-Meyer test to identify blood at the crime scene • Perform a hemagglutination assays to determine suspects and crime scene blood types

RELATED POWERPOINT: Do You Have What It Takes to Solve a Crime?

Cat. #194 Forensics Enhancement Techniques

In this experiment, students will act as detectives following the aftermath of a drug bust involving gang warfare over territory. Reagents that are routinely used as a first screen will be utilized to detect

simulated blood and DNA. In addition, biological materials will be recovered from splatters, blood trajectory, and small droplets of simulated human materials.

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FORENSICS: FINGERPRINT ANALYSIS

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FINGERPRINT MAZE

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ANALYZING FINGERPRINTS

Fingerprints are divided into three general patterns referred to as loops, whorls, and arches. Loops are the most common of fingerprint patterns, representing about 60% of the population. Around 30% of the population has whorls. Arches are the least common of the three general patterns, representing about 10% of all fingerprints. A typical LOOP pattern displays one or more ridges that enter from one side of the print, curve like a loop, and exit from the same side (Figure 1). A WHORL pattern has at least one ridge that makes a complete circle. Many times, the ridges form concentric circles (Figure 2). ARCHES display ridges that enter from one side of the fingertip and exit from the other side. Arches are further subdivided into plain or tented forms. PLAIN ARCHES are wavelike, gently rising in the center of the arch pattern (Figure 3). In contrast, the TENTED ARCH has a sharp cone like pattern at the center of the fingertip (Figure 4).

Figure 1: Loop

Figure 2: Plain Whorl

Figure 3: Plain Arch

Figure 4: Tented Arch

ANALYZE the fingerprints below and DETERMINE if it is a loop, whorl, plain arch, or tented arch pattern. WRITE in your answers on the lines below each fingerprint.

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SPOT THE DIFFERENCES

EXAMINE these two fingerprints and FIND the differences between them. CIRCLE at least 10 differences.

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SPOT THE DIFFERENCES WHAT A MESS! There was a break-in at school and this classroom is a disaster! However, someone has tampered with the evidence at the scene. The proof is in the photos. INVESTIGATE and CIRCLE at least 10 differences between the photo on this page and the photo on the next page.

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SPOT THE DIFFERENCES

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DNA

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DNA PRODUCTS AND RESOURCES:

RELATED EXPERIMENTS

RELATED RESOURCES EDVOTEK INSTRUCTIONAL VIDEO: How Do We Extract DNA From Cells?

Cat. #S-75 Do Onions, Strawberries and Bananas Have DNA? Your students can construct DNA models and then extract DNA from onions, strawberries or bananas. You provide the fruit or vegetables and 95-100% isopropyl alcohol, your students extract DNA.

Learn about living organisms and DNA

EDVOTEK LIVE VIDEO: The Discovery of DNA

• Students construct double-stranded DNA models and ex- plore nucleotide pairing • A classroom spooling demonstration lets students see DNA and introduces this technique • Finally, student extract and spool fruit and vegetable DNA in two hands-on experiments

Cat. #119 Genes in a Tube™

Teach your students how to extract and spool their own DNA in this exciting and easy activity. Students can transfer their DNA to a tube that can be used as a pendant on a necklace!

RELATED POWERPOINT: The Discovery of DNA

Learn about DNA’s structure and biochem- istry

• Understand the biology behind DNA isolation methods • Spool and stain your own DNA using our new and improved isolation method • See and wear this incredible molecule with the included Genes in a Tube™ necklaces

Cat. #EVT-077 DNA Structure Model Teach DNA structure with our interactive model!

With this exciting model your students make base pairs and combine them to make a model of the DNA double helix.

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STRUCTURE OF DNA

Choose different colors for each component and then color in the worksheet.

Base pair

5'

3'

T

A

S

Nitrogen Bases

Color

S

Base pair

Adenine (purine)

A

Guanine (purine)

C

G

G

S

Cytosine (pyrimidine)

C

S

Thymine (pyrimidine)

T

C

Color

Sugar-Phosphate Backbone

G

S

deoxyribose (sugar)

S

S

Hydrogen bonds

phosphate

Nucleotide

T

A

Phosphate

Nitrogen base

S

S

Sugar

Hydrogen bonds 5’ = Five prime end 3’ = Three prime end

C

G

S

S

T

A

S

S

C

G

S

S

3'

5'

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CREATE A 3-D MODEL OF DNA

Students can work in groups of 2-4.

ASSESSMENT:

REQUIRED MATERIALS:

The instructor should evaluate each DNA model before the students consume the candy. Alternatively, students photo- graph their final products for submission. The photo is print - ed, the component parts of DNA are labeled, and the photo is entered into the student’s lab notebook.

Toothpicks (wood or plastic)

• Bag of multicolored soft candy (Gumdrops, gummy bears, or marshmallows work well. Must have four colors) • Bag of licorice sticks • Digital Camera or Cell Phone (optional) PROCEDURE: 1. If students intend to eat the candy after the experiment, be sure they thoroughly clean their hands before creating the model. Plastic wrap or aluminum foil can be placed over desk- tops to create a clean surface.

2. Each group will receive the following items in a plastic bag:

a. Four colors of gummy candy represent the nucleotide bases. Each group should receive at least six candies of each color. Assign a nucleotide to each of the four colors of gummy candy.

i.

Adenine =_____________________

ii. Thymine =_____________________

iii. Cytosine =_____________________

iv. Guanine =_____________________

b. Licorice sticks represent the sugar-phosphate backbone. Each group should receive two. c. Toothpicks represent the base pairing interactions between nucleotides. Each group should receive at least toothpicks. 3. Using a toothpick, skewer two of the gummy candies. Be sure to follow base pairing rules (A=T, G=C). Repeat until all of the gummy candy is used. 4. Attach one piece of licorice to each side of the toothpick. The base pair toothpicks should be added to the licorice in random order. The resulting DNA duplex should look like a ladder, with the licorice as the rails and the nucleotides as the rungs. 5. Pick up the ladder at each end. Carefully twist the DNA model so that it forms a double helix.

DISCUSSION QUESTION: Record the base pair sequence of your DNA molecule. Why is the order of the nucleotides important?

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DNA WORD SEARCH

PIKNHTHYMINEFBPTELOMEREBK POLYMERASEAAHMVPYRIMIDINE TRNATRPAGVCXYPRIOIBYDICQD ANUCLEOTIDEHYPRIMASENPRDM NGYBWZFBPYUEDCOLLINSAUIIV QHAPLOIDSEQUENCEETUQDRCPU AHTYZKGMOQCXLSRXPFYHEIKLF MDNRZEOOLEICHROMOSOMENUOC OHWEYWUJIJQZXJAQOWOWDENIC KMUPWADBAQFMUEFNIGVNVFFDQ AUVLSDZQGMNJTRANSCRIPTION ZNVIBENNUCLEICACIDGSVIWMK ARJCINHCVPBFNOCPXOCAEMARM KDYAOIUOYBTIUEZQNTTQNUTAO ILBTTNMHDTZUCZBUUOGKTTSLC IRAIEEIRSAOIUELYKBUJEAOLC CHSOCULRNOHSEKNEGZTIRTNEC LETNHIEFBANEINITDMCDSICLZ MLKONMHVPLRTKNNURSZNAOBEH ZIWMOOXRGESHAPETIOGGHNQCT AXIILYAOAFHPAGNDQRMGEBALD YAQEOREPLICATIONNRDEFNPNV JUYRGFRANKLINTUSFMWNRUOXX MKCMYGUANINEYIIJCKBEHEDMU RSMCEKGQNHELICASETHTXFTCE TRANSCRIPTION

PURINE REPLICATION POLYMERASE SEQUENCE

TELOMERE DIPLOID OKAZAKI PRIMASE DNA

NUCLEOTIDE CENTROMERE CYTOSINE MUTATION ADENINE VENTER

GUANINE COLLINS THYMINE

HELIX GENE

NUCLEIC ACID REPLICATION

TRNA CRICK MRNA WATSON BIOTECHNOLOGY PYRIMIDINE CHROMOSOME

HELICASE FRANKLIN HAPLOID GENOME ALLELE

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DNA FINGERPRINTING

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DNA FINGERPRINTING PRODUCTS AND RESOURCES:

RELATED EXPERIMENTS

RELATED RESOURCES EDVOTEK LIVE VIDEO: Micropipetting Basics

Cat. #S-45 Pipetting by Numbers:

STEAM Pipetting Practice Help your students master the fun- damental biotechnology technique of micropipetting while creating a colorful, science-themed classroom poster as well as their own artwork.

• Set your classroom up for a year of experimental success by mastering micropipetting • Learn best practices, key parts, and the importance of accu- racy & precision • Engage students with a STEAM wet lab based on “paint by numbers” • Create a colorful, science-themed classroom poster

EDVOTEK INSTRUCTIONAL VIDEO: Preparing an Agarose Gel for Electrorphoresis

Cat. #101 Principles and Practice of Agarose Gel Electrophoresis

EDVOTEK INSTRUCTIONAL VIDEO: Performing Agarose Gel Electrorphoresis

Demonstrate how electrophoresis separates molecules on the basis of size and charge. A safe, colorful, fast and simple way to teach a technique that will engage your students.

Explore how gel electrophoresis separates molecules

• Learn about macromolecules, electromotive force, and molecule sorting • Load and run samples and watch as they separate, migrate at different rates, and even travel in opposite directions! • A hands-on and STEM-focused introduction to this funda- mental biotechnology

QUICK GUIDE: Agarose Electrophoresis

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AGAROSE GEL ELECTROPHORESIS

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ELECTROPHORESIS WORD SEARCH EBHXRGTRPBYBEGXTPKAB BMSPIRTSKCIUQISH0EDZ GMYPPBGXW0RDRILGWTBT RC0ZPLASMIDTSEBIE0MI CNRCNWC0QTAEGXXERVAB FGBNLELHLMRWRKNWSDLC SHYXUENGA0VZSFZRUE0C XTDBCCG0HMKPWHNAPXEA VUHURNLPIABAKDLLPKQF WNLHIU0ECTUEDRJULNUK NE0DBRVRILCFRXECYCAI FIASTAINPCZIDYEEIRAZ N0LCTLSX0QAZRLELKIEN LBEKAAATWPSCJTG0ACCN ZLADNTWMNYRMIJSMGKLV EQDNRAIIUBGGIDYEA00V GEPADERGLUMYVJQWRWCG RBYTJSUFHREFFUBJ0APA DMICR0PIPETTEMRYSNBY YMLLEWW0DD0VTDBDEDTP

EDVOTEK FRANKLIN LADDER NUCLEIC ACID MOLECULAR WEIGHT ELECTROPHORESIS DNA STAIN

DYE QUICKSTRIPS

BANDS BUFFER WATSON CRICK PLASMID PCR RESTRICTION ENZYME LAMBDA COMB MATRIX

GEL LOADING MICROPIPETTE POWER SUPPLY TRAY WELL BIOMOLECULE

CHAMBER AGAROSE

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SCIENCE LAB REPORT

POSING QUESTION:

RESPONDING VARIABLE:

MANIPULATED VARIABLE:

HYPOTHESIS:

CONTROLLED VARIABLE:

PROCEDURE:

OBSERVATIONS:

CONCLUSION:

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PCR

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PROGRAMMING THE THERMAL CYCLER

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TRANSFORMATION

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TRANSFORMATION

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GFP STRUCTURE

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Aequorea victoria

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ELISA

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ELISA WORD SEARCH NSNYW0QWASHBUFFERQDA A00EDENUETARTSBUSIIL SCITS0NGAIDLACIDEMCK METTNEBHKNIP0UYETMAA IFCCCJVIELTGIAMTAUCL CTE0EEMITAWISTSRBNII RETLNRT0TNVSTEBVUELN 0PEIHDIENAAYTAFJCSYE TIDGNKADD0TYCITXNYCP IPCHVDIRNCCIRHMIISIH TRITARIUYNILLAAFVTL0 EENCRBMRAAEN0AMIPEAS RFEHLMTNEUNMENUINMSP PSGAIJGXICVTYGAQRE0H LN0ISELISATXIZ0LRPNA AARNRALLERGENBNMGHIT TR0PLAN0LCYL0P0E0FMA ETUDEKNILEMYZNEDPRAS SZLSTANDARDCURVEYRHE BVFEDIX0REPNEG0RDYHC

ELISA MICROTITER PLATES ANTIGEN

INCUBATE HRP ENZYME ALLERGEN PREGNANCY TEST EPITOPE QUANTITATIVE QUALITATIVE STANDARD CURVE CONTROL

POLYCLONAL MONOCLONAL

CHROMOGENIC DETECTION FLUOROGENIC DETECTION PEROXIDASE ALKALINE PHOSPHATASE DIRECT INDIRECT SANDWICH IMMUNOASSAY ENZYME LINKED LMMUNOSORBENT ASSAY

PRIMARY ANTIBODY S ECONDARY ANTIBODY SUBSTRATE MEDICAL DIAGNOSTICS HYDROGEN PEROXIDE ABTX AMINOSALICYLIC ACID TRANSFER PIPET WASH BUFFER

HEAVY CHAIN LIGHT CHAIN ANTIGEN BINDING SITE IMMUNE SYSTEM

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CHROMATOGRAPHY

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CHROMATOGRAPHY WORD SEARCH

PIKNHTHYMINEFBPTELOMEREBK POLYMERASEAAHMVPYRIMIDINE TRNATRPAGVCXYPRIOIBYDICQD ANUCLEOTIDEHYPRIMASENPRDM NGYBWZFBPYUEDCOLLINSAUIIV QHAPLOIDSEQUENCEETUQDRCPU AHTYZKGMOQCXLSRXPFYHEIKLF MDNRZEOOLEICHROMOSOMENUOC OHWEYWUJIJQZXJAQOWOWDENIC KMUPWADBAQFMUEFNIGVNVFFDQ AUVLSDZQGMNJTRANSCRIPTION ZNVIBENNUCLEICACIDGSVIWMK ARJCINHCVPBFNOCPXOCAEMARM KDYAOIUOYBTIUEZQNTTQNUTAO ILBTTNMHDTZUCZBUUOGKTTSLC IRAIEEIRSAOIUELYKBUJEAOLC CHSOCULRNOHSEKNEGZTIRTNEC LETNHIEFBANEINITDMCDSICLZ MLKONMHVPLRTKNNURSZNAOBEH ZIWMOOXRGESHAPETIOGGHNQCT AXIILYAOAFHPAGNDQRMGEBALD YAQEOREPLICATIONNRDEFNPNV JUYRGFRANKLINTUSFMWNRUOXX MKCMYGUANINEYIIJCKBEHEDMU RSMCEKGQNHELICASETHTXFTCE

CELLULOSE MATRIX GEL FILTRATION MOLECULAR SIEVE MOLECULAR WEIGHT FRACTION COLLECTION STATIONARY PHASE MOBILE PHASE FRIT

SIZE EXCLUSION PORES BEADS SEPARATION PEAK ION EXCHANGE CHROMATOGRAPHY COLUMN MOLECULE PROTEINS RNA DNA ELUTION BUFFER SPECTROPHOTOMETER FRACTIONATION RANGE

ELUTION PACKING

BED VOLUME VOID VOLUME SLURRY RING STAND

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ECO

EXPLORER

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ECO EXPLORER WORD SEARCH MGOCDECOMPOSERRJEWLT GMWGCJHRUYFTGGAPDLOR AGESHAIAAAAJPFHDHRUC NAELWTRBIODIVERSITYO XAVGTSRBLGNPAPKWSGSN GKPRYYSAOROECOLOGYRS BRVPZPNUNNRSWRQSWBEU HUEZHCQISSCEKLEXARUM WJFEFOCVTTPYDCECOQSE TAFTNPTWDRAICUDJYYER ONTOSHCOWHOIRLCUGCKO XBNEOWOOSAXGNAEEKVLW YWIORDTUMYTLEATKISEE GLTPDSWNSPNEJNBIIQAA EFRDZIHEHEOTROCIOLLM NVOTGPFEBMGSHTIYLNDY FJGLFEOADWRATEAYCIAA VAESYTHRJCWOSISBDLTN ENNDCTMCVUBBTANILFEY PCQAAYYRNRZPFZLGSEKA

BIODIVERSITY GREENHOUSE GAS DECOMPOSER NITROGEN TRANSPIRATION WATER TABLE SUSTAINABILITY CARBON CYCLE OXYGEN PHOTOSYNTHESIS

WATERSHED COMPOSTING RECYCLE

CONSUMER FOOD WEB NITROGEN CYCLE ECOLOGY REUSE REDUCE

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WE LOVE SCIENCE!

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PROTEIN ELECTROPHORESIS

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PROGRAMMING THE DIGITAL WATER BATH

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STEM

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STEAM

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GARDENING

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FOOD

SCIENCE

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METEOROLOGY

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