Writing and Publishing Scientific Articles
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The following figure illustrates a complex concept:
Figure 3. A model for exon junction complex (EJC) assembly. EJC assembly is initiated as early as the H complex, when REF and SRp20 become associated with the 5' exon. Additional EJC components join the complex before exon ligation. As the splicing reaction proceeds through exon ligation and mRNP release, at least 3 additional proteins become closely associated with the RNA, while other factors may remain with the spliceosome. Association of some EJC proteins becomes less stable after mRNP release. Proteins identified by cross-linking are named according to their apparent molecular weights, whereas p32/REF and p25/SRp20 were identified by denaturing immunoprecipitation. Proteins lacking a molecular-mass designation (bottom section) were shown to be present in the C complex or spliced mRNP by mass spectrometry or Western blotting. (Reprinted with permission from Reichert VL et al. 5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly. Genes Dev 16:2778 – 2791, 2002; Cold Spring Harbor Laboratory Press © 2002.)
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