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Preliminary Steps in Writing a Scientific Manuscript
Do not worry about the format of your outline; you are the only one who has to be able to follow it. Here is a sample outline on a basic science topic—really just a list of things to mention or describe in the paper and notes to yourself.
X Gene Expression Required for A and Angiogenesis
Introduction Process A is essential for angiogenesis and so may be target for cancer therapy Johnson & colleagues’ original study of process A in 3 cell lines Lei et al. confirmed their conclusions in some cell lines but not others We think it depends on whether cells express X gene (cite AACR abstract) Materials and Methods Sources of cell lines (ATCC, Manassas, VA, for all—except WRDCELL, which we got from Tom at Baylor) PCR protocols, primers (did we buy control or make it?—ask Margaret) In situ hybridization conditions Statistics (ask John the statistician to write that) Results Use all figures from last departmental presentation except gene map Table of expression levels in all cell lines Best in situs Discussion Conclusion: X gene expression is necessary for the A process How our study differed from Johnson and why it supports Lei et al. Possible implications for cancer therapy But we need to test this in tumor samples, which we are already doing
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