GC-KOL-Article-Bio-Emulation_SA

Histo-Anatomic Development

Fig 1 Bell Stage: Histo-differentiation is the process of transforming a mass of similar looking epithelial cells into morphologically and functionally distinct components (enamel/dej complex/dentin). Morpho- differentiation is the process whereby individual tooth buds attain recognizable shapes (incisor vs molar).

METHODOLOGY

In order to ascertain the morphologic relationship between the exterior surface of the enamel layer and the DEJ, the teeth were submerged in 10% hydrochloric acid (HCl, Mallinckrodt Baker, Inc.,

4 Fig 2 Enamel is the densely mineralized brittle yet hard outer shell of the tooth that envelopes/engulfs the softer dentin core; carbonate-rich hydroxyapatite crystals are arranged in enamel rods. Dentin, conversely, is a collagen rich apatite reinforced bio-composite that is resilient yet tougher than enamel and similar at the nanostructural level to bone. It has a unique structural architecture consisting of dentinal tubules, surrounded by peritubular dentin cylinders of randomly oriented apatite crystallites.

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