Gene signature of acquired resistance to statins
Statin resistance signature derived from in vitro model
In vivo validation
A.
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Significant (FDR < 0.05) NS
15
10
5
0
Fig. 2: Acquired resistance to fluvastatin causes restorative metabolic reprogramming. ( A & B). Heatmap showing the genes that map to steroid biosynthesis and terpenoid backbone biosynthesis in the fluvastatin resistant MCF10.AT-R cells, including ACAT1 (SOAT1) and ACAT2. ( C ). Bar diagrams show qPCR validation where over expression of genes (including ACAT1/ SOAT1 and ACAT2 statin resistance gene panel) correlates tumor outcome in fluvastatin treated SV40C3TAgmice. The Y axis depicts the fold changes of average gene expression that was calculated by using the ΔΔ Ct method after normalizing with ribosomal protein L19. Red color in bar diagram represents the fold changes were significant and gray represents non- significant (NS) changes at an FDR of < 5%
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