Results
Exercise at 8 and 16 m/min does not impact on PDAC growth, spreading and desmoplasia
Exercise at 8 and 16 m/min impacts on CAF subtype distribution in PDAC
Exercise at 8 and 16 m/min reduces the expression of CAF-related cytokines in PDAC
Exercise at 8 and 16 m/min reduces CAF abundance in PDAC
Day 32
Whole tumor
Tumor mass
iCAFs
+
NG2
CAFs
✱
1.5
Il1b
Tnf
✱
Tgfb1
✱
80000
40
✱
✱
2.0
2.5
2.0
✱
✱✱
1.0
60000
30
2.0
1.5
1.5
40000
20
1.5
0.5
1.0
1.0
1.0
20000
10
0.5
0.5
0.0
0.5
0
0
0.0
0.0
0.0
Peritoneal carcinomatosis
apCAFs
+
myCAFs
α SMA
CAFs
20
p=0.08
0.095
FACS-isolated CAFs
40000
15
Il6 ✱
100
15
✱
p=0.056
90
30000
3
✱
10
10
Fibroblasts PDPN + CD45 - EpCAM - CD31 -
80
Single cell dissociation
20000
2
5
70
5
10000
60
0
1
0
0
50
Live cells
0
Desmoplasia
myCAF/iCAF ratio
Total CAFs
Cxcl12
Masson's trichrome
Col1a1
Hyaluronic acid
25
0.054
0.08
0.07
✱✱
30
3
2.0
40
15
0.058
20
1.5
30
15
20
2
10
✱
10
1.0
20
1
10
5
5
0.5
10
0
0.0
0
0
0
0
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