Biocatalytic reactions of Organosilyl Ethers using Silicatein-α Chisom Egedeuzu 1,2 Matteo Trande 1,2 , Emily Sparkes 1,2 , Rachel Kettles 1,2 , Yuqing Lu 1,2 , Lu Shin Wong 1,2 1 Manchester Institute of Biotechnology, University of Manchester, UK, 2 Department of Chemistry, University of Manchester, UK email: chisom.egedeuzu@manchester.ac.uk Silicatein-α is an enzyme found in spicules of marine sponges with a catalytic triad similar to cathepsin L and it is able to catalyse the condensation of silicon-oxygen bonds, which these organisms use to polymerise silicic acid (dissolved silicates) into inorganic silica for incorporation into their skeletons [1]. Our laboratory has shown recently that silicatein-α from Suberites domuncula (the prototypical member of this family), is able to catalyse both bond hydrolysis and condensation for a range of model organosilyl ethers (i.e. compounds containing C– Si–O moieties), which alludes to the potential application of these enzymes in organosilicon chemistry [2,3,4]. Here, we report our latest results investigating the catalysis of silicatein-α in the context of synthetic chemistry. Specifically, we demonstrated the roles of the active site residues in catalysis (Si-O hydrolysis and silica formation); and a survey of silyl ether bond condensations from the corresponding alcohols and silanols. References 1. K. Shimizu and D. E. Morse, Methods Enzymol. , 2018, 605 , 429-455. 2. E. I. Sparkes, C. S. Egedeuzu, B. Lias, R. Sung, S. A. Caslin, S. Y. Tabatabaei Dakhili, P. G. Taylor, P. Quayle, L. S. Wong, Catalysts , 2021, 11 , 879. 3. S. Y. Tabatabaei Dakhili, S. A. Caslin, A. S. Faponle, P. Quayle, S. P. de Visser, L. S. Wong, Proc. Natl. Acad. Sci. , 2017, 114 , E5285-E5291. 4. E. I. Sparkes, R. A. Kettles, C. S. Egedeuzu, N. L. Stephenson, S. A. Caslin, S. Y. Tabatabaei Dakhili, L. S. Wong, Biomolecules , 2020, 10 , 1209.
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