Investigating the interactions between Manganese Oxide Nanoparticles and key components of cellular membranes Travis Issler, Kevin Sule, and Elmar J. Prenner University of Calgary, Canada Nanotechnology offers a wide range of applications and can be extremely useful in both industrial and clinical settings. With the growing interest in nanomaterials, it is imperative to determine their impacts on biological systems. Specifically, lipid – nanoparticle interactions are a relevant research area that requires more attention due to potential consequences at the membrane level. Metallic nanoparticles offer a platform for a broad range of applications in both of these settings. Their unique properties compared to pure metal forms; size, shape, and surface charge can alter the biological behaviour of the particles [1,2]. Manganese oxide nanoparticles (MnO NPs) in particular, have been used in the manufacturing of batteries and can act as catalysts and electrochemical materials [3]. Recently, MnO nanoparticles have been demonstrated as suitable contrast agents in magnetic resonance imaging [4]. Possibly providing a less toxic alternative to the historically used gadolinium chelates known to be associated with nephrogenic systemic fibrosis [5]. However, toxicological studies report that metal nanoparticles may induce cellular distress via the production of reactive oxygen species, and even via direct damage to cellular membranes [6,7]. Our studies showcase the impacts of MnO nanoparticle exposure at the level of the cell membrane. Using biophysical techniques, alterations in membrane lipid packing dynamics, lateral membrane organization, fluidity, and permeability in the absence and presence of MnO nanoparticles can be assessed. We determined that MnO NPs caused drastic changes in lipid monolayer stability and lateral monofilm disorganization. References 1. Sobańska, Z., Roszak, J., Kowalczyk, K., & Stępnik, M. (2021). Applications and Biological Activity of Nanoparticles of Manganese and Manganese Oxides in In Vitro and In Vivo Models. Nanomaterials 2021, Vol. 11, Page 1084 , 11 (5), 1084. https://doi.org/10.3390/NANO11051084 2. Jeevanandam, J., Barhoum, A., Chan, Y. S., Dufresne, A., & Danquah, M. K. (2018). Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein Journal of Nanotechnology , 9 (1), 1050. https:// doi.org/10.3762/BJNANO.9.98Khan, S. A. (2020). 3. Metal nanoparticles toxicity: role of physicochemical aspects. Metal Nanoparticles for Drug Delivery and Diagnostic Applications , 1–11. https://doi.org/10.1016/B978-0-12-816960-5.00001-XCai, X., Zhu, Q., Zeng, Y., Zeng, Q., Chen, X., & Zhan, Y. (2019). 4. Manganese Oxide Nanoparticles As MRI Contrast Agents In Tumor Multimodal Imaging And Therapy. International Journal of Nanomedicine , 14 , 8321. https://doi.org/10.2147/IJN.S218085Thomsen, H. S., Morcos, S. K., Almén, T., Bellin, M. F., Bertolotto, M., Bongartz, G., Clement, O., Leander, P., Heinz-Peer, G., Reimer, P., Stacul, F., van der Molen, A., & Webb, J. A. (2013). 5. Nephrogenic systemic fibrosis and gadolinium-based contrast media: Updated ESUR Contrast Medium Safety Committee guidelines. European Radiology , 23 (2), 307–318. https://doi.org/10.1007/S00330-012-2597-9/TABLES/5Manke, A., Wang, L., & Rojanasakul, Y. (2013). 6. Mechanisms of nanoparticle-induced oxidative stress and toxicity. BioMed Research International , 2013 . https://doi.org/10.11 55/2013/942916Stoimenov, P. K., Klinger, R. L., Marchin, G. L., & Klabunde, K. J. (2002). 7. Metal oxide nanoparticles as bactericidal agents. Langmuir , 18 (17), 6679–6686. https://doi.org/10.1021/LA0202374/ASSET/ IMAGES/LARGE/LA0202374F00006.JPEG
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