Fluorescence-based strategies for monitoring/mapping biologically relevant species among bacteria: a new orientation Kai-Cheng Yan 1 ,Jordan E. Gardiner 1 , Adam C. Sedgwick 2 , Naing Thet 1 , Rachel A. Heylen 1 , Tony D. James 1,4 , A. Toby A. Jenkins 1 , Xiao-Peng He 3,5 1 Department of Chemistry, University of Bath, UK, 2 Chemistry Research Laboratory, University of Oxford, UK, 3 Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Shanghai, China, 4 School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, China, 5 National Center for Liver Cancer, the Second Military Medical University, Shanghai, China Multifunctional small-molecular sensors have been developed to monitor biomarkers in bacterial cells that are indicative of important biological/pathological processes during survival/reproduction/infection. The sensors contain fluorophores that are chemically linked to responsive linkers that quench the fluorescence of the fluorophore through fluorescence resonance energy transfer (FRET). Upon the activation of by a corresponding biomarker, the responsive linkers undergoes cleavage and releases the free fluorophore to recover the fluorescence signal. A TCF-based long-wavelength probe was constructed to catch and detect mechanism-related biomarkers among a range of bacterial species. Significantly, the probe was successfully used to monitor in both planktonic cells and biofilm models. References 1. Sedgwick, A. C. 1 ; Yan, K.-C. 1 ; Mangel, D. N. 1 ; Shang, Y.; Steinbrueck, A.; Han, H.-H.; Brewster II, J. T.; Hu, X.-L.; Snelson, D. W.; Lynch, V. M.; Tian, H.; He, X.-P.; Sessler, J. L. J.Am. Chem. 2. Soc. , 2021 , 143 , 1278. Yan, K.-C. ; Patenall, B. L.; Gardiner, J. E.; Heylen, R. A.; Thet, N.; He, X.-P.; Sedgwick, A. C.; James, T. D.; Jenkins, A. T. A. Chem. 3. Commun. , 2022 , 58 , 13103.Zhang, C. 1 ; Shi, D.-T. 1 ; Yan, K.-C. 1 ; Sedgwick, A. C.; Chen, G.-R.; He, X.-P.; James, T. D.; Ye, B.; Hu, X.-L.; Chen, D. Nanoscale , 2020 , 12 , 23234. 4. Thomas, B. 1 ; Yan, K.-C. 1 ; Hu, X.-L.; Donnier-Marechal, M.; Chen, G.-R.; He, X.-P.; Vidal, S. Chem.Soc. Rev. , 2020 , 49 , 593. 5. Yan, K.-C. ; Sedgwick, A. C.; Zang, Y.; Chen, G.-R.; He, X.-P.; Li, J.; Yoon, J.; James, T. D. Small Methods , 2019 , 3 , 1900013. Yan, K.-C. ; Steinbrueck, A.; Sedgwick, A. C.; James, T. D. Front.Sens. , 2021 , 2 , 731928. Hu, X.-L. 1 ; Shang, Y. 1 ; Yan, K.-C. 1 ; Sedgwick, A. C.; Gan, H. Q.; Chen, G.-R.; He, X.-P.; James, T. D.; Chen, D. J.Mater. 6. Chem. B , 2021 , 9 , 3640.Gwynne, L.; Williams, G. T.; Yan, K.-C. ; Patenall, B. L.; Gardiner, J. E.; He, X.-P.; Maillard, J.-Y.; James, T. D.; Sedgwick, A. C.; Jenkins, A. T. A. Biomater.Sci. , 2021 , 9 , 4433.Hu, X.-L. 1 ; Kwon, N. 1 ; Yan, K.-C. ; Sedgwick, A. C.; Chen, G.-R.; He, X.-P.; James, T.-D.; Yoon, J. Adv.Funct. 7. Mater. , 2020 , 30 , 1907906.
P33
© The Author(s), 2023
Made with FlippingBook Learn more on our blog