Gulf of Alaska | Climate and Oceanography
Interlaboratory validation of an enzyme linked immunosorbent assay for domoic acid in blue mussels ( Mytilus edulis ) at the Alutiiq Pride Marine Institute Presenter: Allison Carl , acarl@crrcalaska.org, Chugach Regional Resources Commision
Annette Jarosz , annette@alutiiqprideak.org, Alutiiq Pride Marine Institute Dustin Carl , dustin@crrcalaska.org, Chugach Regional Resources Commision Emily Mailman , emily@alutiiqprideak.org, Alutiiq Pride Marine Institute Shannon Atkinson , shannon.atkinson@alaska.edu, University of Alaska Fairbanks Kendall Mashburn , klmashburn@alaska.edu, University of Alaska Fairbanks Raven Cunningham , raven@crrcalaska.org
Amnesic shellfish poisoning (ASP) is a potentially fatal syndrome caused by domoic acid (DA), which is produced by diatoms in the genius Pseudo-nitzschia. Currently, there is not a state run harmful algal bloom (HAB) monitoring program for subsistence and recreational harvesters in Alaska, putting users at great risk of illness and even death. Regional Tribal networks are taking the initiative to collect critical phytoplankton and shellfish toxin data that can be used to inform subsistence harvesters of real-time toxin risk levels at key shellfish harvest sites. The Chugach Regional Resources Commission (CRRC) is a Tribal consortium representing the seven Tribes in the Prince William Sound, Lower Cook Inlet, and Lower Copper River regions of Alaska. CRRC operates the Alutiiq Pride Marine Institute (APMI) in Seward; as part of its Chugach Regional Ocean Monitoring program, APMI has worked to validate the commercially available enzyme-linked immunosorbent assay (ELISA) (Eurofins) for detection of DA in blue mussels ( Mytilus edulis ). Samples were collected from Afognak Beach in Seward after a Pseudo-nitzschia bloom. Through collaboration with the University of Alaska Fairbanks (UAF) College of Fisheries and Ocean Sciences (CFOS), DA was extracted from M. edulis tissue and toxin levels were measured in both CRRC and UAF CFOS laboratories, outputs were then tested for accuracy and parallelism, which both met adequate requirements for validation. APMI plans to use this assay for both regular monitoring of shellfish toxins and future research applications.
Alaska Marine Science Symposium 2023 105
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