Not all unsatisfactory results, however, originate from biological complexity. A substantial proportion arise from routine process issues. Transcription errors, procedural drift (e.g. enrichment ratios, incubation times or temperatures) and occasional equipment problems were all highlighted by a recent AXIO survey and are well recognised across PT programmes . These represent correctable weaknesses that may not appear during routine QC, but become evident under PT challenge conditions. Although PT is sometimes criticised for not driving long-term improvement, later analyses show this often reflects staff turnover rather than limitations in PT design . Without mechanisms to retain learning between rounds, gains are often lost and performance can remain static. Taken together, these patterns emphasise the need for structured, end-to-end root-cause investigation supported by performance tracking over time. Trend review – particularly when viewed by matrix, strain and analyst within PORTAL – allows laboratories to distinguish isolated events from persistent issues, and to implement targeted actions including staff retraining, SOP refinement and instrument verification. PT therefore remains a central mechanism for benchmarking capability, developing analyst competency, and driving continuous improvement across a wide range of methods and matrices. Incorporating atypical strains and low-level challenges, as required under ISO 17043, ensures that PT exercises reflect the realities of foodborne-pathogen detection and support sustained strengthening of laboratory workflows.
AXIO Proficiency Testing – a trusted partner for microbiology testing
With over 40 years of experience, AXIO Proficiency Testing supports laboratory performance across food, water and environmental testing. The AXIO microbiology programme covers key quality control analytes, spanning routine pathogens and indicator organisms alongside less commonly assessed targets such as Shigella and psychrotrophic bacteria. Salmonella PT forms a core part of the portfolio, with well-characterised strains included across more than 30 food, feed and environmental matrices . These span commonly tested commodities as well as more challenging matrices, enabling laboratories to assess performance across different workflows and matrix types. easi - tab ™ complements PT through ISO 17034-certified quantitative microbiology tablets, including Salmonella -formats, supporting method verification, routine quality control and laboratory training. Visit lgcstandards.com/AXIO to get involved, or speak to one of our PT experts at axiopt@lgcstandards.com .
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lgcstandards.com/AXIO
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