WITH EMALEE MANN, JESSIE BOULOS, AND DR. ANGELA ALEXANDER-BRYANT, DEPARTMENT OF BIOENGINEERING
Glioblastoma multiforme (GBM) is a rare grade IV glioma tumor that affects the central nervous system. Current treatment methods include tumor resection, chemotherapy, and radiation, all resulting in low prognosis. As new therapeutics are being developed, the cellular model by which they are tested should reflect the in vivo tumor environment for better translatability. Three-dimensional models, such as spheroids, can better mimic the GBM tumor environment, unlike the traditional two-dimensional cell models. This study aims to characterize in vitro models for GBM treatments using U87MG spheroids and compare the gene expression of both two-dimensional and three- dimensional models. Western blots and RT-qPCR (Real-Time quantitative PCR) were used to compare the expressions of genes overly expressed in glioblastoma for both 2D cells and 3D spheroids. Future studies include further spheroid characterization and gene expression analysis in 2D and 3D models. Following characterization, U87MG spheroids will be used as an improved in vitro model to investigate a new treatment for GBM, novel co-loaded hydrogels for combination treatment. The Characterization of Three-Dimensional Spheroids for Glioblastoma In Vitro Modeling
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