04 - Follow That Flush: Using Biotechnology for Early Disease Detection
Experiment Results:
RESULTS TABLE
Meaning
Molecular Weight 6751, 3652, 2827, 1568, 1118, 825, 630
Lane
Sample
Used to approximate DNA size
DNA Standard Marker
1
Sample 1 - Treatment Plant
Both Norovirus and E. coli are present in the sample
48502 bp, 4282 bp
2
Sample 2 - Community Center
4282 bp
3
E. coli is present in the sample
Sample 3 - University Dorms
Norovirus is present in the sample
48502 bp
4
Sample 4 - Downtown
48502 bp, 4282 bp
5
Both Norovirus and E. coli are present in the sample
Sample 5 - Regional Airport
No bands
6
Neither Norovirus nor E. coli are present in the sample
*NOTE– In most cases, PCR is not used to amplify sequences over 5000 base pairs. A larger band was used here to produce a clear difference in size between samples.
Related Experiment:
Follow That Flush: Using Biotechnology to Stop Outbreaks For 8 Gels. Wastewater testing is a public health tool that helps scientists track specific diseases in a com - munity. In this experiment, students use electropho- resis to test simulated water samples for bacteria and viruses. By learning how wastewater monitoring works, students see how biotechnology helps protect public health.
Cat. #922 Cat. #922-S
Features FlashBlue™ Stain Features SYBR® Safe Stain
46
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