EDVOTEK® BIOTECHNOLOGY BOOTCAMP
Bacterial Transformation and Transformation Efficiency
STORY TILES: TRANSFORMATION PROTOCOL Below are story tiles depicting a common lab procedure for creating competent cells (bacteria cells that can take in exogenous DNA). Using the lab instructions provided below as a guide, CUT OUT the cards and REORDER them in the correct sequence. FILL IN the numerical sequence in the left box of each story tile for future reference or PASTE onto a new sheet of paper in the correct order.
plasmid solution
42°C
Approx. 5 colonies
1. ADD Calcium Chloride to a sterile test tube and place on ice for 2 minutes. 2. Using a sterile loop, TRANSFER 5 well isolated bacteria colonies to the test tube containing Calcium Chloride.
3. TWIST the loop between your fingers to free the cells. 4. RESUSPEND the bacteria cells by pipetting up and down. 5. ADD the plasmid solution to the tube. 6. INCUBATE on ice for 10 minutes. Just one tube. 7. TRANSFER to a warm water bath, incubate for 45 seconds.
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